Human Integrin beta 5 Biotinylated Antibody

Catalog #: BAF3824
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF3824

Integrin beta 5 in MCF‑7 Human Cell Line.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin beta 5 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin beta 5 in Western blots. In Western blots, less than 5% cross-reactivity with Integrin beta 1, -beta 2, -beta 3, recombinant mouse Integrin beta 4, -beta 6, and -beta 7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta 5
Gly24-Asn719
Accession # P18084

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Integrin beta 5

Flow Cytometry

2.5 µg/10⁶ cells

MG‑63 human osteosarcoma cell line

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Integrin beta 5 in MCF‑7 Human Cell Line. Integrin beta 5 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Integrin beta 5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to transmembrane proteins. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 5

Integrin beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin alpha _V. Integrin alpha _V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin beta 5, respectively.

Entrez Gene IDs

3693 (Human); 16419 (Mouse); 257645 (Rat); 102123619 (Cynomolgus Monkey)

Alternate Names

FLJ26658; Integrin beta 5; integrin beta-5; integrin, beta 5; ITGB5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Integrin beta 5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Integrin &alphav&beta5 heterodimer is a specific marker of human pancreatic beta cells
Authors: JV Schiesser, T Loudovaris, HE Thomas, AG Elefanty, EG Stanley
Scientific Reports, 2021-04-15;11(1):8315.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, ICC, IHC