Human Integrin beta 8 Antibody

Catalog #: MAB47752 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB47752-100
MAB47752-SP

Detection of Integrin beta 8 in MG-63 Human Cell Line by Flow Cytometry.

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Human Integrin beta 8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin beta 8 in direct ELISAs

Source

Recombinant Monoclonal Rabbit IgG Clone # 2723C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human Integrin alpha V beta 8
Human Integrin alpha V (Phe31-Val992) and Human Integrin beta 8 (Glu43-Arg684)
Accession # NP_002201.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

MG‑63 human osteosarcoma cell line

Immunocytochemistry

3-25 µg/mL

Immersion fixed MG‑63 human osteosarcoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin beta 8 in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Rabbit Anti-Human Integrin beta 8 Monoclonal Antibody (Catalog # MAB2723C, filled histogram) or Rabbit IgG Control Antibody (MAB1050,open histogram) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry

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Integrin beta 8 in MG‑63 Human Cell Line. Integrin beta 8 was detected in immersion fixed MG‑63 human osteosarcoma cell line using Rabbit Anti-Human Integrin beta 8 Monoclonal Antibody (Catalog # MAB47752) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 8

Integrin beta 8 (Integrin beta 8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alpha V to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alpha V beta 8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin beta 8 shares 87% aa sequence identity with mouse and rat Integrin beta 8.

Entrez Gene IDs

3696 (Human); 320910 (Mouse); 362800 (Rat)

Alternate Names

Integrin beta 8; integrin beta-8; integrin, beta 8; ITGB8