Home / ITIH4 / Human ITIH4 Antibody

Human ITIH4 Antibody

Catalog #: AF8157
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF8157
AF8157-SP
Detection of Human ITIH4 by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ITIH4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ITIH4 in direct ELISAs and Western blots.   In direct ELISAs, less than 2% cross-reactivity with recombinant human ITIH1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ITIH4
Glu29-Leu930 (Ile85Asn, Pro698Thr)
Accession # Q14624
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ITIH4 antibody by Western Blot. View Larger

Detection of Human ITIH4 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITIH4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8157) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITIH4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITIH4

The inter-alpha -trypsin inhibitor heavy chain (ITIH4), also known as IHRP (inter-alpha -trypsin heavy chain-related protein), PK-120 (plasma kallikrein-sensitive glycoprotein 120), or Gp120 (glycoprotein 120), is a secreted, 120 kDa, 930 amino acid (aa), N-glycosylated Type II acute phase protein. It is upregulated in response to trauma such as ischemic stroke or myocardial infarction, and functions as a trypsin inhibitor. It is activated by Kallikrein by cleavage between aa 688-689, creating 100 kDa and 35 kDa portions. Mature human ITIH4 shares 66% aa sequence identity with mouse and rat ITIH4. Human isoforms lacking aa 621-650 (914, 900 and 845 aa forms), aa 727-765 and 851-866 (845 aa) and having 14 aa inserted after aa 727 (914 aa) have been described. Proteolytic cleavage by plasma kallikrein produces a 100 kDa fragment that is further processed to 70 kDa, and 35 kDa fragments. An O-glycosylated form is reported in urine, but not in plasma.

Long Name
Inter-alpha-Trypsin Inhibitor Heavy Chain H4
Entrez Gene IDs
3700 (Human); 16427 (Mouse); 54404 (Rat)
Alternate Names
DKFZp686G21125; gp120; H4P; heavy chain-like, 1; IHRP; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein); inter-alpha-trypsin inhibitor family heavy chain-related protein; inter-alpha-trypsin inhibitor heavy chain H4; ITIH4; ITI-HC4; ITIHL1; PK120; PK-120; plasma kallikrein-sensitive glycoprotein 120; PRO1851

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human ITIH4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Proteomics changes after negative pressure wound therapy in diabetic foot ulcers
    Authors: Z Jia, L Liu, S Zhang, X Zhao, L Luo, Y Tang, B Shen, M Chen
    Molecular Medicine Reports, 2021-10-05;24(6):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human ITIH4 Antibody

There are currently no reviews for this product. Be the first to review Human ITIH4 Antibody and earn rewards!

Have you used Human ITIH4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review