Human Kallikrein 1 Antibody

Catalog #: MAB2337 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2337
MAB2337-SP

All Kallikrein 1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) KLK-3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, -14, rhHGFA, rhFactorVII, rhFactorXI, rhThrombin or rhUPA-1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 323803

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 1
Ile25-Ser262
Accession # NP_002248

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Kallikrein 1 (Catalog # 2337-SE) under non-reducing conditions only

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Kallikrein 1 (Catalog # 2337-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 1

Kallikrein 1 (KLK1), also known as tissue kallikrein, is a member of human tissue kallikrein family (1). The best known physiological function of KLK1 is the cleavage of kininogen to release the vasoactive kinin peptide (bradykinin or lysyl-bradykinin), which regulates vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation (2). In addition, KLK1 may play a role in angiogensis and tumorigenesis (2). Human KLK1 precursor contains a signal peptide (residues1 to 18), a short pro peptide (residues 19 to 24) and a mature chain (residues 25 to 262). The purified rhKLK1 contains the mature chain and the propeptide from human KLK5. After being activated by thermolysin, rhKLK1 is active against a fluorogenic peptide substrate described above.

References

Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Chao, J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1577.

Entrez Gene IDs

3816 (Human); 16612 (Mouse)

Alternate Names

EC 3.4.21; EC 3.4.21.35; glandular kallikrein 1; hK1; Kallikrein 1; kallikrein 1, renal/pancreas/salivary; kallikrein serine protease 1; kallikrein-1; Kidney/pancreas/salivary gland kallikrein; KLK1; Klk6; KLKR; Tissue kallikrein