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Human Kallikrein 7 Antibody

Catalog #: MAB2624
3 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2624
MAB2624-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Kallikrein 1, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, or 15 is observed.
Source
Monoclonal Mouse IgG2A Clone # 333931
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 7
Glu23-His252
Accession # P49862
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Kallikrein 7 (Catalog # 2624-SE) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 7 (Catalog # 2624-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Kallikrein 7 (2 µg/mL, Catalog # 2624-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 5.5 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 7

Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length Kallikrein 7 consists of 253 amino acids (aa), with a signal peptide (aa 1-22), short pro peptide (aa 23-29) and mature chain (aa 30-252) (1). Predominantly expressed in the skin, a major physiological function of Kallikrein 7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for Kallikrein 7 is Kallikrein 5, another member of the human tissue Kallikrein family. Along with Kallikrein 14, these three Kallikreins form a proteolytic cascade in the stratum corneum (5).

References
  1. Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
  2. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
  3. Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
  4. Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
  5. Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.
Entrez Gene IDs
5650 (Human); 23993 (Mouse)
Alternate Names
EC 3.4.21; EC 3.4.21.117; hK7; hSCCE; kallikrein 7 (chymotryptic, stratum corneum); Kallikrein 7; kallikrein-related peptidase 7; KLK7; protease, serine, 6; PRSS6; SCCEkallikrein-7; Serine protease 6; signal protein; Stratum corneum chymotryptic enzyme

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Citations for Human Kallikrein 7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice
    Authors: K Kidana, T Tatebe, K Ito, N Hara, A Kakita, T Saito, S Takatori, Y Ouchi, T Ikeuchi, M Makino, TC Saido, M Akishita, T Iwatsubo, Y Hori, T Tomita
    EMBO Mol Med, 2018-03-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin.
    Authors: Bin L, Kim BE, Hall CF
    J. Invest. Dermatol., 2011-07-14;131(11):2213-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid.
    Authors: Morizane S, Yamasaki K, Kabigting FD, Gallo RL
    J. Invest. Dermatol., 2010-01-21;130(5):1297-306.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Development, ICC

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Human Kallikrein 7 Antibody
By Anonymous on 04/26/2018
Application: WB Sample Tested: SK-OV-3 human ovarian adenocarcinoma cell line Species: Human