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Human KIR2DL1/KIR2DS5 Fluorescein-conjugated Antibody

Catalog #: FAB1844F
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1844F-025
FAB1844F-100
Detection of KIR2DL1/KIR2DS5 in Human Blood Lymphocytes by Flow Cytometry.
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Citations (13)
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Human KIR2DL1/KIR2DS5 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DL1/KIR2DS5 on transfected cells. It does not stain cells transfected with KIR2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG1 Clone # 143211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL1/KIR2DS5
Accession # P43626
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DL1/KIR2DS5 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of KIR2DL1/KIR2DS5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human KIR2DL1/KIR2DS5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1844F) or (B) Mouse IgG1 Isotype Control Antibody (Catalog # IC002F) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DL1/KIR2DS5

KIR2DL1 is one of several immunoglobulin-like receptors expressed on NK cells that bind MHC class I molecules and transmit inhibitory signals. KIR2DL1 is specific for HLA-C alleles with Asn77 and Lys80.

Alternate Names
CD158a; KIR2DL1/KIR2DS5

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Citations for Human KIR2DL1/KIR2DS5 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Engagement of TRAIL triggers degranulation and IFN gamma production in human natural killer cells
    Authors: Johannes Höfle, Timo Trenkner, Nadja Kleist, Vera Schwane, Sarah Vollmers, Bryan Barcelona et al.
    EMBO reports
  2. Human Cytomegalovirus Infection Enhances NK Cell Activity In Vitro
    Authors: Grzegorz Terszowski
    Transplant Direct, 2016-06-17;2(7):e89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. New insights on the natural killer cell repertoire from a thorough analysis of cord blood cells
    Authors: P Rettman, C Willem, G David, R Riou, N Legrand, J Esbelin, A Cesbron, D Senitzer, K Gagne, C Retière
    J Leukoc Biol, 2016-04-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Tissue-Specific Education of Decidual NK Cells.
    Authors: Sharkey A, Xiong S, Kennedy P, Gardner L, Farrell L, Chazara O, Ivarsson M, Hiby S, Colucci F, Moffett A
    J Immunol, 2015-08-28;195(7):3026-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The Human NK Cell Response to Yellow Fever Virus 17D Is Primarily Governed by NK Cell Differentiation Independently of NK Cell Education.
    Authors: Marquardt N, Ivarsson M, Blom K, Gonzalez V, Braun M, Falconer K, Gustafsson R, Fogdell-Hahn A, Sandberg J, Michaelsson J
    J Immunol, 2015-08-17;195(7):3262-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. 2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells.
    Authors: Dunphy S, Guinan K, Chorcora C, Jayaraman J, Traherne J, Trowsdale J, Pende D, Middleton D, Gardiner C
    Genes Immun, 2015-05-07;16(5):301-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Uptake of CCR7 by KIR2DS4(+) NK cells is induced upon recognition of certain HLA-C alleles.
    Authors: Pesce S, Carlomagno S, Moretta A, Sivori S, Marcenaro E
    J Immunol Res, 2015-04-16;2015(0):754373.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. KIR/HLA interactions negatively affect rituximab- but not GA101 (obinutuzumab)-induced antibody-dependent cellular cytotoxicity.
    Authors: Terszowski G, Klein C, Stern M
    J Immunol, 2014-05-02;192(12):5618-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Expansion of CMV-mediated NKG2C+ NK cells associates with the development of specific de novo malignancies in liver-transplanted patients.
    Authors: Achour, Abla, Baychelier, Florence, Besson, Caroline, Arnoux, Armelle, Marty, Michel, Hannoun, Laurent, Samuel, Didier, Debre, Patrice, Vieillard, Vincent
    J Immunol, 2013-12-04;192(1):503-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2012-12-12;43(2):480-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Human Immunodeficiency Virus Type 1 Infection Is Associated with Increased NK Cell Polyfunctionality and Higher Levels of KIR3DL1+ NK Cells in Ugandans Carrying the HLA-B Bw4 Motif.
    Authors: Eller MA, Koehler RN, Kijak GH
    J. Virol., 2011-03-16;85(10):4802-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
    Authors: Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG
    J. Exp. Med., 2010-12-20;208(1):13-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Pro- and Anti-Inflammatory Cytokines in the Context of NK Cell-Trophoblast Interactions
    Authors: V Mikhailova, P Grebenkina, E Khokhlova, A Davydova, Z Salloum, E Tyshchuk, V Zagainova, K Markova, I Kogan, S Selkov, D Sokolov
    International Journal of Molecular Sciences, 2022-02-21;23(4):.

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