Human KLF12 Antibody

Catalog #: MAB9097 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9097
MAB9097-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KLF12 Antibody Summary

Species Reactivity

Human

Specificity

Detects human KLF12 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1230C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived human KLF12
Asn2-Val402
Accession # Q9Y4X4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

1-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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KLF12 in HEK293 Human Cell Line. KLF12 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rabbit Anti-Human KLF12 Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB9097) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry

Detection of KLF12 antibody in MG-63 Human Cell Line antibody by Flow Cytometry.

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Detection of KLF12 in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Rabbit Anti-Human KLF12 Monoclonal Antibody (Catalog # MAB9097, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF12

Krueppel-like factor 12 is a protein that in humans is encoded by the KLF12 gene. The protein encoded by this gene confers strong transcriptional repression to the AP-2-alpha gene, a developmentally-regulated transcription factor active during vertebrate development and carcinogenesis. KLF12 has been reported as a novel tumor-suppressor gene whose loss of function is associated with anoikis resistance. Three isoforms have been found for this gene.

Long Name

Kruppel-Like Factor 12

Entrez Gene IDs

11278 (Human); 16597 (Mouse); 306110 (Rat)

Alternate Names

AP-2rep transcription factor; AP2REP; AP-2rep; AP2REPAP-2 repressor; HSPC122; KLF12 zinc finger transcriptional repressor; KLF12; Krueppel-like factor 12; Kruppel-like factor 12; Transcriptional repressor AP-2rep