Human L-Selectin/CD62L Antibody

Catalog #: AF728
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF728
AF728-SP

Neutralization of L Selectin/CD62L Fc Chimera by Human L Selectin/CD62L Antibody

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Citations (3)

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Human L-Selectin/CD62L Antibody Summary

Species Reactivity

Human

Specificity

Detects human L‑Selectin in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human L-Selectin

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human L-Selectin/CD62L (Catalog # ADP2)

Neutralization

Measured by its ability to neutralize L-Selectininduced adhesion of LS180 human colorectal adenocarcinoma cells. The Neutralization Dose (ND50) is typically 0.500 - 6.00 µg/mL in the presence of 10 ug/mL Recombinant Human L-Selectin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Neutralization of L Selectin/CD62L Fc Chimera by Human L Selectin/CD62L Antibody Human L Selectin/CD62L Antibody (Catalog # AF728) neutralizes Recombinant Human L Selectin/CD62L Fc Chimera (Catalog # 728-LS) induced adhesion of LS180 human colorectal adenocarcinoma cells. The ND50 for this effect is typically 0.500-6.00 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: L-Selectin/CD62L

Human L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L‑Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90-100 kDa. L‑Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L‑Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble L‑Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Alternate Names

CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human L-Selectin/CD62L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1.
Authors: Sinha RK, Mage RG
Dev. Comp. Immunol., 2004-06-01;28(7):829-41.
Species: Rabbit
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-Fr
Synthetic glycoprotein mimics inhibit L-selectin-mediated rolling and promote L-selectin shedding.
Authors: Mowery P, Yang ZQ, Gordon EJ, Dwir O, Spencer AG, Alon R, Kiessling LL
Chem. Biol., 2004-05-01;11(5):725-32.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Lipid raft compartmentalization of urokinase receptor signaling in human neutrophils.
Authors: Sitrin RG, Johnson DR, Pan PM, Harsh DM, Huang J, Petty HR, Blackwood RA
Am. J. Respir. Cell Mol. Biol., 2003-08-21;30(2):233-41.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot