Human Legumain/Asparaginyl Endopeptidase Antibody Summary
Val18-Tyr433
Accession # Q99538
Applications
Human Legumain Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Legumain/Asparaginyl Endopeptidase
Legumain is a lysosomal cysteine protease whose activity is found in several tissues tested (1, 2). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (3). Over-expression of Legumain in tumors is significant for invasion/metastasis (4). Also known as Asparaginyl Endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of the cleavage, which result in the removal of the pro peptides in both C- and N-termini (5). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha 1 and thymosin alpha 11, two acidic peptides with immunoregulatory properties (6‑8). Human Legumain is synthesized as a 433 amino acid precursor with a signal peptide (residues 1‑17). The pro enzyme (residues 18‑433) was expressed with an N-terminal His tag. This activity of Legumain can be inhibited by rhCystatins C and E/M and recombinant mouse Cystatin C (R&D Systems, Catalog # 1196-PI, 1286-PI and 1238-PI, respectively).
- Chen, J.-M. et al. (1997) J. Biol. Chem. 272:8090.
- Tanaka, T. et al. (1996) Cytogenet. Cell Genet. 74:120.
- Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
- Liu, C. et al. (2003) Cancer Res. 63: 2957.
- Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
- Chen, J.M. et al. (2001) Biol. Chem. 382:777.
- Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
- Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.
Product Datasheets
Citation for Human Legumain/Asparaginyl Endopeptidase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Simvastatin inhibits glucose metabolism and legumain activity in human myotubes.
Authors: Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H
PLoS ONE, 2014-01-08;9(1):e85721.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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