Human LIF Antibody

Catalog # Availability Size / Price Qty
MAB250-SP
MAB250-100
MAB250-500
Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody.
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Product Details
Citations (4)
FAQs
Supplemental Products
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Human LIF Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIF in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-2, rhIL-3, rhIL-4, rhIL-6, rhIL-7, rhIL-8, rhG-CSF, rhGM-CSF, rhOSM, rhTGF-beta 1, rhTNF-alpha, rhTNF-beta, recombinant mouse (rm) IL-1 beta, rmIL-3, rmIL-4, rmIL-5, rmIL-6, rmIL-7, rmGM-CSF, bovine (b) FGF acidic, bFGF basic, human (h) PDGF, porcine (p) PDGF, hTGF-beta 1, pTGF-beta 1.2, or pTGF-beta 2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 9824
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human LIF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human lung
Neutralization
Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.06-0.2 µg/mL in the presence of 1.5 ng/mL Recombinant Human LIF. Human LIF Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) is recommended for neutralization.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. View Larger

Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 1.5 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB250). The ND50 is typically 0.06-0.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIF

LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells (17).

References
  1. Van-Vlasselaev, P. et al. (1992) Prog. Growth Factor Res. 4:337.
  2. Gough, N.M. (1992) Growth Factors 7:175.
  3. Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
  4. Gillett, N.A. et al. (1993) Growth Factors 9:301.
  5. Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
  6. Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
  7. Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
  8. Williams, R.L. et al. (1988) Nature 336:684.
  9. Baumann, H. et al. (1989) J. Immunol. 143:1163.
  10. Marshall, M.K. et al. (1994) Endocrinology 135:1412.
  11. Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
  12. Davis, S. et al. (1993) Science 260:18054.
  13. Gearing, D.P. et al. (1991) EMBO J. 10:28395.
  14. Gearing, D.P. et al. (1991) Science 255:1434.
  15. Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
  16. Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.
Long Name
Leukemia Inhibitory Factor
Entrez Gene IDs
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names
CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI

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Citations for Human LIF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS.
    Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
    J. Biol. Chem., 2011-07-22;286(39):34271-85.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007-09-11;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  3. Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
    Authors: Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A
    Mol. Hum. Reprod., 2004-03-25;10(5):313-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes.
    Authors: Hess S, 2019, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A
    7046, 2001-10-01;44(10):2392-401.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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