Human LIF Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 1.5 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB250). The ND50 is typically 0.06-0.2 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIF
LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells (17).
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- Gough, N.M. (1992) Growth Factors 7:175.
- Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
- Gillett, N.A. et al. (1993) Growth Factors 9:301.
- Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
- Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
- Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
- Williams, R.L. et al. (1988) Nature 336:684.
- Baumann, H. et al. (1989) J. Immunol. 143:1163.
- Marshall, M.K. et al. (1994) Endocrinology 135:1412.
- Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
- Davis, S. et al. (1993) Science 260:18054.
- Gearing, D.P. et al. (1991) EMBO J. 10:28395.
- Gearing, D.P. et al. (1991) Science 255:1434.
- Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
- Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.
Product Datasheets
Citations for Human LIF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS.
Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
J. Biol. Chem., 2011-07-22;286(39):34271-85.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
Blood, 2007-09-11;110(13):4319-30.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
Authors: Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A
Mol. Hum. Reprod., 2004-03-25;10(5):313-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes.
Authors: Hess S, 2019, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A
7046, 2001-10-01;44(10):2392-401.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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