Human LILRA6/CD85b Antibody

Catalog #: MAB8656 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8656
MAB8656-SP

Detection of LILRA6/CD85b in HEK293 Human Cell Line Transfected with Human LILRA6/CD85b and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRA6/CD85b Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRA6/CD85b in direct ELISA. Detects HEK293 cells transfected with LILRA6/CD85b but not irrelevant transfectants.

Source

Monoclonal Mouse IgG₁ Clone # 921330

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line-derived recombinant human LILRA6/CD85b
Met1-Asn447
Accession # Q6PI73

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LILRA6/CD85b in HEK293 Human Cell Line Transfected with Human LILRA6/CD85b and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human LILRA6/CD85b and eGFP were stained with either (A) Mouse Anti-Human LILRA6/CD85b Monoclonal Antibody (Catalog # MAB8656) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Flow Cytometry

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Detection of LILRA6/CD85b in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human LILRA6/CD85b Monoclonal Antibody (Catalog # MAB8656, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA6/CD85b

LILRA6 (Leukocyte immunoglobulin-like receptor subfamily A, member 6) is also known as ILT5 and CD85b. The leukocyte immunoglobulin-like receptors (LILR) are a family of receptors possessing extracellular immunoglobulin domains. They are also known as ILTs and can exert immunomodulatory effects on a wide range of immune cells. LILRA6 is a highly polymorphic surface molecule that is one of the targets of dendritic cell-reactive antibodies.

Long Name

Leukocyte Immunoglobulin-like Receptor A6

Entrez Gene IDs

79168 (Human); 18726 (Mouse)

Alternate Names

7M1; CD85b; CD85b;ILT5;ILT8;ILT-8;LILRB3;LILRB6; ILT8; LILRA6; Pira3