Human LILRB3/CD85a/ILT5 Antibody

Catalog #: MAB18061 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB18061-100
MAB18061-SP

Detection of LILRB3/CD85a/ILT5 in human neutrophils with CD15 costain cells by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB3/CD85a/ILT5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRB3/CD85a/ILT5 in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1057017

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human LILRB3/CD85a/ILT5
Gly24-Glu443
Accession # O75022

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human neutrophils with CD15 costain and PBMCs with CD14 costain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LILRB3/CD85a/ILT5 in human neutrophils with CD15 costain cells by Flow Cytometry. Human neutrophils with CD15 costain cells were stained with Mouse Anti-Human/Mouse SSEA‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2155P) and either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 Monoclonal Antibody (Catalog # MAB18061) or (B) Mouse IgG₁ Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of LILRB3/CD85a/ILT5 in PBMC with CD14 costain cells by Flow Cytometry. PBMC with CD14 costain were stained with either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 Monoclonal Antibody (Catalog # MAB18061) or (B) Mouse IgG₁ Isotype Control (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB3/CD85a/ILT5

Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. Subfamily A members are activating receptors that lack ITIMs and signal through association with FcR gamma (1, 2). Mature LILRB3 is a highly polymorphic 85‑95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3). Alternative splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human LILRB3 share 55% aa sequence identity within common regions of their ECDs. LILRB3 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors (4-6). Triggering of LILRB3 inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5, 7). On osteoclast precursors, LILRB3 ligation inhibits RANK L/TRANCE or M-CSF induced differentiation (8). LILRB3 can also bind to ligands exposed on necrotic tumor cells (9). Both PIR-B and LILRB3 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (3). R&D Systems in-house testing indicates that LILRB3 binds to
Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (10). In the mouse CNS, PIR-B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (11). Upon binding to MAG, PIR-B associates with TrkB and NGF R/p75 in cerebellar granule neurons (12).

References

Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
Nakayama, M. et al. (2007) J. Immunol. 178:4250.
Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1174.
Sloane, D.E. et al. (2004) Blood 104:2832.
Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
Mori, Y. et al. (2008) J. Immunol. 181:4742.
Jones, D.C. et al. (2016) Oncotarget PMID 26769854.
Zheng, J. et al. (2012) Nature 485:656.
Atwal, J.K. et al. (2008) Science 322:967.
Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.

Entrez Gene IDs

11025 (Human)

Alternate Names

CD85 antigen-like family member A; CD85a antigen; CD85a; HL9Immunoglobulin-like transcript 5; ILT5; ILT-5; ILT5CD85a; Leukocyte immunoglobulin-like receptor 3; leukocyte immunoglobulin-like receptor subfamily B member 3; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; member 3; Monocyte inhibitory receptor HL9; PIRB