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Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Antibody

Catalog #: FAB24251F
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB24251F-025
FAB24251F-100
Detection of LILRB4/CD85k/ILT3 in Human PBMCs by Flow Cytometry.
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Citations (3)
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Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ILT3/CD85k in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with with recombinant human (rh) ILT5 is observed. No cross-reactivity with rhILT1, rhILT2, rhILT4, rhILT7, rhILT11, rhLIR-6 or rhLIR-8 is observed.
Source
Monoclonal Mouse IgG2A Clone # 293623
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ILT3/CD85k
Pro17-His257
Accession # ABM86208
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LILRB4/CD85k/ILT3 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of LILRB4/CD85k/ILT3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL-3 Ra/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB24251F) or (B) Mouse IgG2AFluorescein Isotype Control (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRB4/CD85k/ILT3

Immunoglobulin-Like Transcript 3 (ILT3), also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
Entrez Gene IDs
11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)
Alternate Names
CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4

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Citations for Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Protein kinase CK2 enables regulatory T cells to suppress excessive TH2 responses in vivo.
    Authors: Ulges A, Klein M, Reuter S, Gerlitzki B, Hoffmann M, Grebe N, Staudt V, Stergiou N, Bohn T, Bruhl T, Muth S, Yurugi H, Rajalingam K, Bellinghausen I, Tuettenberg A, Hahn S, Reissig S, Haben I, Zipp F, Waisman A, Probst H, Beilhack A, Buchou T, Filhol-Cochet O, Boldyreff B, Breloer M, Jonuleit H, Schild H, Schmitt E, Bopp T
    Nat Immunol, 2015-01-19;16(3):267-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
    Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
    PLoS ONE, 2011-07-25;6(7):e22504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. DC-SIGN ligation greatly affects dendritic cell differentiation from monocytes compromising their normal function.
    Authors: Svajger U, Obermajer N, Anderluh M, Kos J, Jeras M
    J. Leukoc. Biol., 2011-03-29;89(6):893-905.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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