Human LIN-41 Antibody Summary
Val386-Phe868
Accession # Q2Q1W2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human LIN‑41 by Western Blot. Western blot shows lysates of BG0IV human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LIN-41 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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LIN‑41 in BG01V Human Stem Cells. LIN-41 was detected in immersion fixed BG01V human embryonic stem cells using Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human LIN‑41 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for LIN-41 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIN-41
LIN-41 (lineage-41; also TRIM/tripartite motif-containing protein 71) is a 94 kDa (predicted) cytosolic member of the RBCC (RING finger, B-Box Coiled-Coil) family of proteins. It is expressed in developing limbs, DRG and branchial arches and apparently regulates the timed expression of proliferation and differentiation-associated genes. Human LIN-41 is 868 amino acids (aa) in length. It contains a RING-type zinc finger region (aa 12-95), a His-rich segment (aa 146‑157), two B Box zinc fingers that bind DNA (aa 194-314), a coiled-coil motif that binds protein (aa 391-427), one filamin repeat and six NHL repeats that regulate gene translation (aa 593‑868). Over aa 386-868, human LIN-41 is 97%, 95%, and 94% aa identical to canine, mouse, and chicken LIN-41, respectively.
Product Datasheets
Citation for Human LIN-41 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.
Authors: Bronevetsky, Yelena, Villarino, Alejandr, Eisley, Christop, Barbeau, Rebecca, Barczak, Andrea J, Heinz, Gitta A, Kremmer, Elisabet, Heissmeyer, Vigo, McManus, Michael, Erle, David J, Rao, Anjana, Ansel, K Mark
J Exp Med, 2013-02-04;210(2):417-32.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot
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