Human Lipocalin-2/NGAL Antibody Summary
Gln21-Gly198
Accession # P80188
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Lipocalin‑2/NGAL in Human Pancreas. Lipocalin-2/NGAL was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, HT-29 human colon adenocarcinoma cell line, and human bone marrow tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Human Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1757) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipocalin-2/NGAL
Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3-5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).
- Flower, D.R. et al. (1994) FEBS Lett. 354:7.
- Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426.
- Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
- Devireddy, L.R. et al. (2001) Science 293:829.
- Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
- Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
- Flo, T.H. et al. (2004) Nature 432:917.
- Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
Product Datasheets
Citations for Human Lipocalin-2/NGAL Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Social transmission of inflammation in mice
Authors: Castany, S;Rosa, PB;Shionoya, K;Blomqvist, A;Engblom, D;
Brain, behavior, and immunity
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Molecular Profiling of Tissue Samples with Chronic Rejection from Patients with Chronic Lung Allograft Dysfunction: A Pilot Study in Cystic Fibrosis Patients
Authors: F Lunardi, DI Abbrescia, L Vedovelli, F Pezzuto, F Fortarezza, GM Comacchio, V Guzzardo, P Ferrigno, M Loy, C Giraudo, AS Fraia, E Faccioli, F Braccioni, E Cozzi, D Gregori, GM Verleden, F Calabrese, FP Schena, F Rea
Biomolecules, 2023-01-03;13(1):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
Authors: F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma
Nature Communications, 2021-12-17;12(1):7333.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures
Authors: I Zhang, P Lépine, C Han, M Lacalle-Au, CX Chen, R Haag, TM Durcan, D Maysinger
Cells, 2020-11-07;9(11):.
Species: Human
Sample Types: Cell Lysates, Organoid, Whole Cells
Applications: ICC, IHC, Western Blot -
Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies
Authors: S An, I Raju, B Surenkhuu, JE Kwon, S Gulati, M Karaman, A Pradeep, S Sinha, C Mun, S Jain
Ocul Surf, 2019-04-06;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Reactive astrocytes secrete lcn2 to promote neuron death.
Authors: Bi, Fangfang, Huang, Cao, Tong, Jianbin, Qiu, Guang, Huang, Bo, Wu, Qinxue, Li, Fang, Xu, Zuoshang, Bowser, Robert, Xia, Xu-Gang, Zhou, Hongxia
Proc Natl Acad Sci U S A, 2013-02-19;110(10):4069-74.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Lipocalin 2 expression is associated with aggressive features of endometrial cancer.
Authors: Mannelqvist M, Stefansson I, Wik E, Kusonmano K, Raeder M, Oyan A, Kalland K, Moses M, Salvesen H, Akslen L
BMC Cancer, 2012-05-06;12(0):169.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1.
Authors: Mir SU, Jin L, Craven RJ
J. Biol. Chem., 2012-03-14;287(18):14494-501.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
J. Immunol., 2008-11-15;181(10):7420-7.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Urinary biomarkers predict brain tumor presence and response to therapy.
Authors: Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA
Clin. Cancer Res., 2008-04-15;14(8):2378-86.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9.
Authors: Gupta K, Shukla M, Cowland JB, Malemud CJ, Haqqi TM
Arthritis Rheum., 2007-10-01;56(10):3326-35.
Species: Human
Sample Types: Synovial Fluid
Applications: Western Blot -
Urinary lipocalin-2 is associated with renal disease activity in human lupus nephritis.
Authors: Pitashny M, Schwartz N, Qing X, Hojaili B, Aranow C, Mackay M, Putterman C
Arthritis Rheum., 2007-06-01;56(6):1894-903.
Species: Human
Sample Types: Urine
Applications: Western Blot
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Formalin-fixed lung sections
