Human LMO2 Antibody Summary
Ser2-Ile158
Accession # P25791
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of LMO2 in K562 Human Cell Line by Flow Cytometry. K562 chronic myelogenous leukemia human cell line was stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.
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Detection of LMO2 in Human PBMC by Flow Cytometry Human PBMC were stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.
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LMO2 in K562 Human Cell Line. LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LMO2
LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.
Product Datasheets
Citations for Human LMO2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice
Authors: KI Hirano, H Hosokawa, M Koizumi, Y Endo, T Yahata, K Ando, K Hozumi
Elife, 2021-08-12;10(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP -
A cell-based screening method using an intracellular antibody for discovering small molecules targeting the translocation protein LMO2
Authors: N Bery, CJR Bataille, A Russell, A Hayes, F Raynaud, S Milhas, S Anand, H Tulmin, A Miller, TH Rabbitts
Science Advances, 2021-04-09;7(15):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells
Authors: M Nafria, P Keane, ES Ng, EG Stanley, AG Elefanty, C Bonifer
Cell Rep, 2020-05-26;31(8):107691.
Species: Human
Sample Types:
Applications: ChIP-Seq -
LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage
Authors: VS Stanulovic, P Cauchy, SA Assi, M Hoogenkamp
Nucleic Acids Res., 2017-09-29;45(17):9874-9888.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors
Nat Commun, 2016-11-21;7(0):13396.
Species: Human
Sample Types: Complex Sample Type
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Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential.
Authors: Treanor L, Zhou S, Janke L, Churchman M, Ma Z, Lu T, Chen S, Mullighan C, Sorrentino B
J Exp Med, 2014-03-31;211(4):701-13.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Aberrant induction of LMO2 by the E2A-HLF chimeric transcription factor and its implication in leukemogenesis of B-precursor ALL with t(17;19).
Authors: Hirose K, Inukai T, Kikuchi J, Furukawa Y, Ikawa T, Kawamoto H, Oram SH, Gottgens B, Kiyokawa N, Miyagawa Y, Okita H, Akahane K, Zhang X, Kuroda I, Honna H, Kagami K, Goi K, Kurosawa H, Look AT, Matsui H, Inaba T, Sugita K
Blood, 2010-06-02;116(6):962-70.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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