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Human LNK/SH2B3 Antibody

Catalog #: AF5888
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5888
AF5888-SP
Detection of Human LNK/SH2B3 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LNK/SH2B3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LNK/SH2B3 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LNK/SH2B3 Gln427-Leu575 Accession # Q9UQQ2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human LNK/SH2B3 antibody by Western Blot. View Larger

Detection of Human LNK/SH2B3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, SH-SY5Y human neuroblastoma cell line, Ramos human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LNK/SH2B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LNK/SH2B3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: LNK/SH2B3

LNK (also SH2B adaptor protein 3) is a protein intermediary, or LiNK, between cell surface molecules (TPO R; EPO R; SCFR; TCR) and downstream signaling molecules such as PI3 K, Grb2 and Jak2. Although its predicted MW is 63 kDa, it runs anomalously at 75 kDa in SDS-PAGE. LNK is found in hematopoietic cells, and serves to limit responsiveness to growth factor stimulation. Human LNK is 575 amino acids (aa) in length. It contains an N-terminal Phe-zipper segment that likely serves as a dimerization domain (aa 24-81), a PH domain that may interact with membrane lipids (aa 197-305), and an SH2 domain that binds phosphoTyr (aa 364-462).

Long Name
Lymphocyte Adapter Protein
Entrez Gene IDs
10019 (Human); 16923 (Mouse)
Alternate Names
IDDM20; LNK; LNKSH2B adapter protein 3; Lymphocyte adapter protein; Lymphocyte-specific adapter protein Lnk; SH2B adaptor protein 3; SH2B3; Signal transduction protein Lnk

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Citations for Human LNK/SH2B3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. LNK suppresses interferon signaling in melanoma
    Authors: LW Ding, QY Sun, JJ Edwards, LT Fernández, XB Ran, SQ Zhou, RA Scolyer, JS Wilmott, JF Thompson, N Doan, JW Said, N Venkatacha, JF Xiao, XY Loh, M Pein, L Xu, DW Mullins, H Yang, DC Lin, HP Koeffler
    Nat Commun, 2019-05-20;10(1):2230.
    Species: Human
    Sample Types: Cell Lysates, Tissue Array
    Applications: IHC, Western Blot
  2. LNK (SH2B3): paradoxical effects in ovarian cancer.
    Authors: Ding, L-W, Sun, Q-Y, Lin, D-C, Chien, W, Hattori, N, Dong, X-M, Gery, S, Garg, M, Doan, N B, Said, J W, Xiao, J-F, Yang, H, Liu, L-Z, Meng, X, Huang, R Y-J, Tang, K, Koeffler, H P
    Oncogene, 2014-04-07;34(11):1463-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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