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Human LRRC15 Antibody

Catalog #: MAB11324 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB11324-100
MAB11324-SP
Detection of LRRC15 in HEK293 cells by Flow Cytometry.
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Human LRRC15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRRC15 in direct ELISA.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2944A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human LRRC15
Met1-Gly538
Accession # Q8TF66
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Lysates of Human skin and U‑118‑MG human glioblastoma/astrocytoma cell line
Simple Western
20 µg/mL
Lysates of U-87 MG human glioblastoma/astrocytoma cell line
Flow Cytometry
0.25 µg/106 cells
HEK293 cells transfected with Human LRRC-15 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of LRRC15 in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human LRRC-15 and eGFP were stained with eGFP and either (A) Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Simple Western View Larger

Detection of Human LRRC15 by Simple WesternTM. Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LRRC15 at approximately 104 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot View Larger

Detection of Human LRRC15 by Western Blot. Western blot shows lysates of Human skin and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LRRC15 at approximately ~70-80kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC15

LRRC15 is a type-I 15-leucine-rich repeat containing membrane protein that is located on chromosome 3 at 3q29.  It is a 581 aa membrane protein that belong to the LRR superfamily and lacks an intracellular signaling domain.  Reports indicate that it is highly expressed on cancer-associated fibroblasts in numerous solid tumors, and in the epithelial cancer cell compartment of glioblastoma, sarcomas and melanoma tumors.  LRRC15 functions by regulating cell-cell and cell-extracellular matrix (ECM) interactions, likely partnering with fibronectin, laminin and collagen IV through its extracellular leucine-rich repeats.  LRRC15 is reported in multiple solid tumor types including breast, ovarian and cervical cancers where it exhibits Increased stromal expression (1).  Recent studies indicate that LRRC15 is co-expressed with COL10A1, a gene that is overexpressed in many types of solid tumors, including breast cancer (2).

References
  1. Cancer Res. 2022 March 15; 82(6): 1038. doi:10.1158/0008-5472.CAN-21-0622. 
  2. Biosci Rep. 2020 Feb 28; 40(2): BSR20193286. doi: 10.1042/BSR20193286.
Long Name
Leucine-rich Repeat Containing 15
Entrez Gene IDs
131578 (Human); 74488 (Mouse); 246296 (Rat)
Alternate Names
hLib; leucine rich repeat containing 15; LIB; LRRC15

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