Human Ly6K Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Ly6K in HeLa (positive) and K562 (negative) cells. Ly6K was detected in immersion fixed HeLa (positive) and K562 (negative) cells using Mouse Anti-Human Ly6K Monoclonal Antibody (Catalog # MAB66481) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Ly6K by Western Blot. Western blot shows lysates of human Testes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ly6K Monoclonal Antibody (Catalog # MAB66481) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ly6K at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Preparation and Storage
Background: Ly6K
Product Datasheets
FAQs
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