Human Ly6K Antibody

Catalog #: MAB66481 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB66481-100
MAB66481-SP

Detection of Ly6K in HeLa (positive) and K562 (negative) cells.

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Human Ly6K Antibody Summary

Species Reactivity

Human

Source

Monoclonal Mouse IgG2b Clone # 26304

Formulation

*Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Immunocytochemistry

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Ly6K in HeLa (positive) and K562 (negative) cells. Ly6K was detected in immersion fixed HeLa (positive) and K562 (negative) cells using Mouse Anti-Human Ly6K Monoclonal Antibody (Catalog # MAB66481) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot

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Detection of Human Ly6K by Western Blot. Western blot shows lysates of human Testes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ly6K Monoclonal Antibody (Catalog # MAB66481) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ly6K at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.