Human Lysosomal Pro-X Carboxypeptidase/PRCP Antibody

Catalog #: AF7164 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7164
AF7164-SP

Detection of Human Lysosomal Pro‑X Carboxypeptidase/PRCP by Western Blot.

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All Lysosomal Pro-X Carboxypeptidase/PRCP Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lysosomal Pro-X Carboxypeptidase/PRCP Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lysosomal Pro‑X Carboxypeptidase/PRCP in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lysosomal Pro‑X Carboxypeptidase/PRCP
Met1-His496
Accession # P42785

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Lysosomal Pro‑X Carboxypeptidase/PRCP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Lysosomal Pro-X Carboxypeptidase/PRCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7164) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysosomal Pro-X Carboxypeptidase/PRCP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lysosomal Pro-X Carboxypeptidase/PRCP

PRCP (Prolylcarboxypeptidase; also PCP, lysosomal Pro-X carboxypeptidase and lysosomal carboxypeptidase C) is a lysosomal 57-62 kDa glycoprotein member of the S28 family of proteases. It is expressed by a number of cells, including fibroblasts, macrophages, and endothelial cells. PRCP cleaves a variety of single C‑terminal amino acids (aa) adjacent to a Pro residue, and is known to act on, and inactivate, peptides such as prekallikrein, alpha -MSH and angiotensin II plus III. Human PRCP is apparently synthesized as a prepropeptide that contains a signal sequence (aa 1-21), a short prosequence (aa 22-45) and a 451 aa mature region (aa 46-496). The mature region demonstrates a Ser-carboxypeptidase domain (aa 55-477) that, in itself, possesses an SKS domain that caps the enzyme's catalytic site. The molecule is known to be mannosylated, and to form noncovalent homodimers. There is one splice variant that contains a 21 aa insertion after Lys56. Over aa 1-496, human and mouse PRCP share 77% aa sequence identity.

Entrez Gene IDs

5547 (Human); 72461 (Mouse); 293118 (Rat)

Alternate Names

Angiotensinase C; EC 3.4.16.2; HUMPCP; Lysosomal carboxypeptidase C; lysosomal Pro-X carboxypeptidase; PCPMGC2202; PRCP; Proline carboxypeptidase; prolylcarboxypeptidase (angiotensinase C); prolylcarboxypeptidase isoform 1 preproprotein; Prolylcarboxypeptidase