Human M-CSF APC-conjugated Antibody

Catalog #: IC2161A Datasheet / COA / SDS

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IC2161A

Detection of M‑CSF in Human Macrophages by Flow Cytometry.

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All M-CSF Antibodies

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Human M-CSF APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human M-CSF.

Source

Monoclonal Mouse IgG_2A Clone # 26786

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human M-CSF

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of M-CSF antibody in Human Macrophages antibody by Flow Cytometry.

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Detection of M‑CSF in Human Macrophages by Flow Cytometry. Human macrophages treated with LPS and Recombinant Human TNF-a (Catalog # 210-TA) were stained with Mouse Anti-Human M-CSF APC-conjugated Monoclonal Antibody (Catalog # IC2161A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: M-CSF

M-CSF, also known as CSF-1, is produced by a variety of cell types in either a membrane-anchored or secreted soluble form. It is a glycosylated, disulfide-linked homodimer that binds to the M-CSF receptor expressed by cells of the monocyte-macrophage lineage.

Long Name

Macrophage Colony Stimulating Factor

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Alternate Names

colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930