Human MAD1L1 Antibody

Catalog #: AF4194 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4194
AF4194-SP

Detection of Human MAD1L1 by Western Blot.

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Human MAD1L1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MAD1L1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MAD1L1
Met1-Asp350
Accession # Q9Y6D9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MAD1L1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human MAD1L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4194) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MAD1L1 at approximately 90 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAD1L1

MAD1L1 (mitotic arrest deficient 1-like 1) is a member of the spindle-assembly checkpoint (SAC) which prevents anaphase from occurring until all chromosomes are properly aligned at the metaphase plate. Upon checkpoint activation, MAD1L1 recruits MAD2 to unattached kinetochores, promoting the binding of MAD2 to CDC20, the activator for the anaphase-promoting complex. MAD1L1 has been implicated to have tumor-suppressing functions, but in small-cell lung cancer cell lines, the MAD1L1 gene is the most frequent copy number gain, suggesting a more complex role.

Long Name

MAD1 Mitotic Arrest Deficient-Like 1

Entrez Gene IDs

8379 (Human); 17120 (Mouse); 680006 (Rat)

Alternate Names

hMAD1; HsMAD1; MAD1 mitotic arrest deficient-like 1 (yeast); MAD1; MAD1L1; MAD1-like protein 1; MAD1yeast, homolog-like 1; Mitotic checkpoint MAD1 protein homolog; PIG9; Tax-binding protein 181; TP53I9; tumor protein p53 inducible protein 9; TXBP181; TXBP181MAD1 (mitotic arrest deficient, yeast, homolog)-like 1