Human MCT1/SLC16A1 Antibody

Catalog #: MAB8275 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8275
MAB8275-SP

Detection of MCT1/SLC16A1 in HEK293 Human Cell Line Transfected with Human MCT1/SLC16A1 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MCT1/SLC16A1 Antibody Summary

Species Reactivity

Human

Specificity

Stains human MCT1/SLC16A1 transfected cells but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 882616

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human MCT1/SLC16A1
Met1-Val500
Accession # P53985

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MCT1/SLC16A1 in HEK293 Human Cell Line Transfected with Human MCT1/SLC16A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human MCT1/SLC16A1 and eGFP was stained with either (A) Mouse Anti-Human MCT1/SLC16A1 Monoclonal Antibody (Catalog # MAB8275) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCT1/SLC16A1

Solute Carrier, family 16, member 1 (SLC16A1) encodes Monocarboxylic Acid Transporter 1 (MCT1). MCT1 is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. MCT1 has been found to be important in lactate uptake in cancer cells, and blockade of MCT1 may inhibit lactate-induced HIF-1 activation (1).

References

Sonveaux, P. et al (2012) PLoS ONE 7:e33418.

Long Name

Monocarboxylic Acid Transporter 1 (Solute Carrier Family 16 Member 1)

Entrez Gene IDs

6566 (Human); 20501 (Mouse); 25027 (Rat)

Alternate Names

FLJ36745; HHF7; MCT 1; MCT; MCT1; MCT1MGC44475; member 1; monocarboxylate transporter 1; SLC16A1; Solute carrier family 16 member 1; solute carrier family 16, member 1 (monocarboxylic acid transporter 1)