Human MCT1/SLC16A1 PE-conjugated Antibody

Catalog #: FAB8275P Datasheet / COA / SDS

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FAB8275P

Detection of MCT1/SLC16A1 in HEK293 Human Cell Line Transfected with Human MCT1/SLC16A1 and eGFP by Flow Cytometry.

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Human MCT1/SLC16A1 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human MCT1/SLC16A1 transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 882616

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human MCT1/SLC16A1
Met1-Val500
Accession # P53985

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MCT1/SLC16A1 in HEK293 Human Cell Line Transfected with Human MCT1/SLC16A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human MCT1/SLC16A1 or (B) irrevelant transfectant and eGFP was stained with Mouse Anti-Human MCT1/SLC16A1 PE-conjugated Monoclonal Antibody (Catalog # FAB8275P). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCT1/SLC16A1

Solute Carrier, family 16, member 1 (SLC16A1) encodes Monocarboxylic Acid Transporter 1 (MCT1). MCT1 is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. MCT1 has been found to be important in lactate uptake in cancer cells, and blockade of MCT1 may inhibit lactate-induced HIF-1 activation (1).

References

Sonveaux, P. et al (2012) PLoS ONE 7:e33418.

Long Name

Monocarboxylic Acid Transporter 1 (Solute Carrier Family 16 Member 1)

Entrez Gene IDs

6566 (Human); 20501 (Mouse); 25027 (Rat)

Alternate Names

FLJ36745; HHF7; MCT 1; MCT; MCT1; MCT1MGC44475; member 1; monocarboxylate transporter 1; SLC16A1; Solute carrier family 16 member 1; solute carrier family 16, member 1 (monocarboxylic acid transporter 1)