Human Megalin/LRP2 PE-conjugated Antibody Summary
Pro3510-Lys3964
Accession # P98164
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Megalin/LRP2 in NSO cells transfected with Human LRP2 cells by Flow Cytometry. NSO cells transfected with Human LRP2 were stained with Mouse Anti-Human Megalin/LRP2 PE‑conjugated Monoclonal Antibody (Catalog # FAB9578P, filled histogram) or irrelevant transfectant. To facilitate intracellular staining, cells were fixed with FC004 and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Megalin/LRP2
Megalin, also known as the low-density lipoprotein receptor-related protein 2 (LRP2), is a large type I transmembrane cell surface protein. This glycoprotein is a multi‑ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney (1). The Megalin protein is critical for the re-uptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling. Mutations in this gene cause Donnai-Barrow Syndrome (DBS) and Facio-Oculoacoustico-Renal Syndrome (FOAR) (1). Megalin is consisting of a 25 amino acid (aa) probable N‑terminal signal peptide sequence, a 4400 aa extracellular region, a 22 aa single transmembrane domain, and a 213 aa C‑terminal cytoplasmic tail. The entire extracellular region is made up of 36 class A motifs of putative ligand-binding domains arranged in four distinct clusters, 16 growth factor repeats separated by 8 YWTD spacer regions, and 1 epidermal growth factor-like repeat (2). The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase (3). The amino acid 3510-3964 encodes the fourth class A motif cluster in human Megalin, termed Megalin C4. Human Megalin C4 shares 77% and 74% identity with mouse and rat Megalin C4.
- Christensen, E. I. and Birn, H. (2002) Nat. Rev. Mol. Cell Biol 3:256.
- Saito, A. et al. (1994) Proc.Natl. Aca. Sci. U. S. A. 91:9725.
- Kantarci, S. et al. (2007) Nat. Genet 39:957.
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