Human mGluR2 Antibody

Catalog #: MAB46762 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB46762-100
MAB46762-SP

Detection of mGLUR2 in HEK293 Human Cell Line Transfected with Human mGLUR2 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human mGluR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human mGluR2 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 421614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human mGluR2
Glu19-Ser498
Accession # Q14416

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffinembedded sections of rat brain (cerebellum)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of mGLUR2 in HEK293 Human Cell Line Transfected with Human mGLUR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human mGLUR2 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human mGLUR2 Monoclonal Antibody (Catalog # MAB46762) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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mGluR2 in Rat Brain. mGluR2 was detected in immersion fixed paraffin-embedded sections of rat brain (cerebellum) using Mouse Anti-Human mGluR2 Monoclonal Antibody (Catalog # MAB46762) at 8 µg/mL for 1 hour at room temperature followed by incubation with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (NL007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of neurons in granular cell layer. Staining was performed using our protocol for Fluorescent IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR2

Metabotropic glutamate receptor 2 (mGluR2) is an 872 aa, predicted 96 kDa multipass G protein coupled inhibitory receptor belonging to group II of the metabotropic glutamate receptor family. It is localized largely on the presynaptic side of glutamatergic and other neurotransmitter synapses in areas of the forebrain. mGluR2 activity is potentially involved in some anxiety disorders. The long N‑terminal extracellular region of human mGluR2 (aa 1‑498) shares 97% aa identity with either mouse or rat mGluR2.

Long Name

Metabotropic Glutamate Receptor 2

Entrez Gene IDs

2912 (Human); 108068 (Mouse); 24415 (Rat)

Alternate Names

GLUR2; glutamate metabotropic receptor 2; glutamate receptor, metabotropic 2; GPRC1B; GPRC1Bmetabotropic glutamate receptor 2; GRM2; mGlu2; mGluR2; MGLUR2glutamate receptor homolog; mGluR7