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Human MIS/AMH DuoSet ELISA

Catalog #: DY1737
2 Citations Datasheet / COA / SDS

Discontinued Product

DY1737 has been discontinued.
View all MIS/AMH products.
Human MIS / AMH ELISA Standard Curve
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Citations (2)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human MIS/AMH DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
93.8 - 6,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MIS / AMH. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human MIS / AMH ELISA Standard Curve View Larger

Human MIS / AMH ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MIS/AMH

Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to Sertoli cells of fetal and postnatal testis, and to granulosa cells of postnatal ovary. MIS is synthesized and secreted as a disulfide-linked homodimeric propeptide. Proteolytic cleavage is required to separate the N-terminal proregion from the C-terminal bioactive peptide. Both fragments remain associated in a non-covalent complex. The C-terminal sequence forms a cysteine knot via seven canonical cysteine residues found in TGF-beta superfamily members.

Long Name:
Mullerian-inhibiting Substance/Anti-Mullerian Hormone
Entrez Gene IDs:
268 (Human); 11705 (Mouse)
Alternate Names:
AMH; MIF; MIS; Muellerian hormone; muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian hormone; Mullerian inhibiting factor; Mullerian inhibiting substance

Citations for Human MIS/AMH DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9
    Authors: L Cook, M Sengelmann, B Winkler, C Nagl, S Koch, U Schlomann, EP Slater, MA Miller, EP von Strand, B Dörsam, C Preu beta er, JW Bartsch
    International Journal of Molecular Sciences, 2022-02-10;23(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Predictors of low ovarian reserve in cART-treated women living with HIV
    Authors: E Merlini, C Tincati, V Sacchi, M Augello, V Bono, ES Cannizzo, M Allegrini, L Gazzola, AD Monforte, AM Marconi, M Ravizza, G Marchetti
    Medicine, 2021-10-01;100(39):e27157.
    Species: Human
    Sample Types: Plasma

FAQs

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