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Human MMP-14/MT1-MMP PE-conjugated Antibody

Catalog #: FAB9181P
1 Citation Datasheet / COA / SDS
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FAB9181P
Detection of MMP‑14/MT1‑MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
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Human MMP-14/MT1-MMP PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).
Source
Monoclonal Mouse IgG2B Clone # 128527
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP
Tyr112-Ala541 (predicted)
Accession # P50281
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MMP-14/MT1-MMP antibody in MDA-MB-231 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MMP‑14/MT1‑MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human MMP-14/MT1-MMP PE-conjugated Monoclonal Antibody (Catalog # FAB9181P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MMP-14/MT1-MMP

MMP-14/MT1-MMP (Matrix Metalloproteinase 14) is a member of the peptidase M10A family of enzymes. As such, it is one of some 25 zinc-dependent endopeptidases that are either soluble or membrane-bound.  MMP-14 is expressed as a 63-64 kDa precursor that undergoes processing to generate either a 60 kDa catalytically-active transmembrane form, or a 55 kDa active soluble form. A number of substrates are reported for MMP-14, including Collagen I, II, and III, Laminin, Endoglin, Muc-1, Pro-MMP-13 and -2, and CD44. Molecules suggested to inhibit MMP-14 include TIMP-2, -3, and -4. Cells known to express MMP-14 include monocytes, platelets, osteoclasts, endothelial cells, fibroblasts, and muscle satellite cells. Over amino acids (aa) 112-284, human MMP-14 shares 98% aa sequence identity with mouse MMP-14.

Long Name
Matrix Metalloproteinase 14/Membrane Type 1 MMP
Entrez Gene IDs
4323 (Human)
Alternate Names
EC 3.4.24; EC 3.4.24.80; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1

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Citation for Human MMP-14/MT1-MMP PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Membrane Type 1 Matrix Metalloproteinase Regulates Monocyte Migration and Collagen Destruction in Tuberculosis.
    Authors: Sathyamoorthy T, Tezera L, Walker N, Brilha S, Saraiva L, Mauri F, Wilkinson R, Friedland J, Elkington P
    J Immunol, 2015-06-19;195(3):882-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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