Human MMP-2 Antibody

Catalog #: MAB902
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB902
MAB902-SP

2 Images

All MMP-2 Antibodies

Product Details

Citations (9)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects pro/active forms of human MMP-2 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP‑9, rhMMP-1, or rhMMP-3 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 36006

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP-2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025) under non-reducing conditions only

Immunohistochemistry

50-100 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

MMP‑2 in Human Ovarian Cancer Tissue. MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP-2 Monoclonal Antibody (Catalog # MAB902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

View Larger

MMP‑2 in Human Ovarian Cancer Tissue. MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP‑2 Monoclonal Antibody (Catalog # MAB902) at 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting;and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name

Matrix Metalloproteinase 2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Alternate Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
Authors: Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
Int. J. Cancer, 2010-03-01;126(5):1095-108.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
Biol. Reprod., 2010-01-14;82(5):968-79.
Species: Human
Sample Types: Whole Cells
Applications: Cell-based ELISA
High matrix metalloproteinase production correlates with immune activation and leukocyte migration in leprosy reactional lesions.
Authors: Teles RM, Teles RB, Amadeu TP, Moura DF, Mendonca-Lima L, Ferreira H, Santos IM, Nery JA, Sarno EN, Sampaio EP
Infect. Immun., 2009-12-14;78(3):1012-21.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
In vitro secretion of matrix metalloprotease 9 is a prognostic marker in childhood acute lymphoblastic leukemia.
Authors: Schneider P, Costa O, Legrand E
Leuk. Res., 2009-09-12;34(1):24-31.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells.
Authors: Ooi YH, Oh DJ, Rhee DJ
Invest. Ophthalmol. Vis. Sci., 2009-05-14;50(11):5259-65.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Urinary biomarkers predict brain tumor presence and response to therapy.
Authors: Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA
Clin. Cancer Res., 2008-04-15;14(8):2378-86.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
pVHL co-ordinately regulates CXCR4/CXCL12 and MMP2/MMP9 expression in human clear-cell renal cell carcinoma.
Authors: Struckmann K, Mertz K, Steu S, Storz M, Staller P, Krek W, Schraml P, Moch H
J. Pathol., 2008-03-01;214(4):464-71.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions.
Authors: Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A
Surgery, 2006-09-01;140(3):454-63.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
Oxidized low-density lipoproteins stimulate extracellular matrix metalloproteinase Inducer (EMMPRIN) release by coronary smooth muscle cells.
Authors: Haug C, Lenz C, Diaz F, Bachem MG
Arterioscler. Thromb. Vasc. Biol., 2004-08-19;24(10):1823-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development