Human MMP-9 Fluorescein-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of MMP‑9 in NS0 Mouse Cell Line Transfected with Human MMP-9 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human MMP-9 was stained with Mouse Anti-Human MMP-9 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC9111F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MMP-9
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.
Product Datasheets
Citations for Human MMP-9 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Plasmatic MMP9 released from tumor-infiltrating neutrophils is predictive for bevacizumab efficacy in glioblastoma patients: an AVAglio ancillary study
Authors: C Jiguet-Jig, S Boissonnea, E Denicolai, V Hein, R Lasseur, J Garcia, S Romain, R Appay, T Graillon, W Mason, AF Carpentier, AA Brandes, L' Ouafik, W Wick, A Baaziz, JP Gigan, RJ Argüello, D Figarella-, O Chinot, E Tabouret
Acta neuropathologica communications, 2022-01-03;10(1):1.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Amphiregulin Regulates Phagocytosis-Induced Cell Death in Monocytes via EGFR and Matrix Metalloproteinases
Authors: C Platen, S Dreschers, LK Reiss, J Wappler, TW Orlikowsky
Mediators Inflamm., 2018-11-04;2018(0):4310419.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
MMP9 integrates multiple immunoregulatory pathways that discriminate high suppressive activity of human mesenchymal stem cells
Authors: C Lavini-Ram, HM Silva, A Soares-Sch, SM Monteiro, LR Ferreira, AP Pacanaro, S Gomes, J Batista, K Faé, J Kalil, V Coelho
Sci Rep, 2017-04-13;7(1):874.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease
Authors: NI Medeiros, RC Fares, EP Franco, GR Sousa, RT Mattos, AT Chaves, MD Nunes, WO Dutra, R Correa-Oli, MO Rocha, JA Gomes
PLoS Negl Trop Dis, 2017-01-24;11(1):e0005284.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
JAK/STAT regulation of Aspergillus fumigatus corneal infections and IL-6/23-stimulated neutrophil, IL-17, elastase, and MMP9 activity
Authors: PR Taylor, S Roy, EC Meszaros, Y Sun, SJ Howell, CJ Malemud, E Pearlman
J Leukoc Biol, 2016-03-31;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Development and characterization of a physiologically relevant model of lymphocyte migration in chronic lymphocytic leukemia.
Authors: Walsby E, Buggins A, Devereux S, Jones C, Pratt G, Brennan P, Fegan C, Pepper C
Blood, 2014-03-17;123(23):3607-17.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
Eur. J. Haematol., 2009-11-17;84(3):239-51.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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