Human/Mouse Angiopoietin-1 Antibody

Catalog # Availability Size / Price Qty
AF923
AF923-SP
Angiopoietin‑1 in Human Prostate Cancer Tissue.
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Product Details
Citations (12)
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Supplemental Products
Reviews (1)

Human/Mouse Angiopoietin-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Angiopoietin-1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1
Ser20-Phe498
Accession # Q5HYA0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Angiopoietin‑1 (Catalog # 923-AN)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Angiopoietin-1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Angiopoietin‑1 in Human Prostate Cancer Tissue. Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Angiopoietin-1 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

Angiopoietin‑1 in Human Prostate. Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Angiopoietin-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Angiopoietin‑1 in Mouse Embryo. Angiopoietin-1 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1).

Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% amino acid sequence identity with mouse Ang-1 and human Ang-2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

References
  1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
  2. Davis, S. et al. (1996) Cell 87:1161.
  3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
  4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
  5. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
Entrez Gene IDs
284 (Human); 11600 (Mouse)
Alternate Names
AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003

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Citations for Human/Mouse Angiopoietin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain
    Authors: YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong
    Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764.
    Species: Mouse
    Sample Types: Protein Lysates
    Applications: Western Blot
  2. Loss of Endothelial Glycocalyx Hyaluronan Impairs Endothelial Stability and Adaptive Vascular Remodeling After Arterial Ischemia
    Authors: G Wang, MR de Vries, WMPJ Sol, AM van Oevere, HC de Boer, AJ van Zonnev, PHA Quax, TJ Rabelink, BM van den Be
    Cells, 2020-03-29;9(4):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. EphA4/Tie2 crosstalk regulates leptomeningeal collateral remodeling following ischemic stroke
    Authors: B Okyere, WA Mills Iii, X Wang, M Chen, J Chen, A Hazy, Y Qian, JB Matson, MH Theus
    J. Clin. Invest., 2020-02-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Impact of heat therapy on recovery after eccentric exercise in humans
    Authors: Kyoungrae Kim, Shihuan Kuang, Qifan Song, Timothy P. Gavin, Bruno T. Roseguini
    Journal of Applied Physiology
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. The Fibrin Cleavage Product B?15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury
    Authors: D Fischer, C Seifen, P Baer, M Jung, C Mertens, B Scheller, K Zacharowsk, R Hofmann, TJ Maier, A Urbschat
    Front Pharmacol, 2018-04-27;9(0):369.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Ang-2 but not Ang-1 expression in perivascular soft tissue tumors
    Authors: Swati Shrestha
    J Orthop, 2016-11-28;14(1):147-153.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers.
    Authors: Baruah P, Lee M, Wilson P, Odutoye T, Williamson P, Hyde N, Kaski J, Dumitriu I
    Br J Cancer, 2015-07-14;113(4):653-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis.
    Authors: Brunckhorst MK, Wang H, Lu R, Yu Q
    Cancer Res., 2010-09-07;70(18):7283-93.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy.
    Authors: Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA
    FASEB J., 2008-05-23;22(8):3010-23.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction.
    Authors: Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard JF, Pouplin S, Boumier P, Vittecoq O, Le Loet X, Boissier MC
    Clin. Immunol., 2007-06-08;124(2):158-64.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  11. Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
    Authors: Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006-05-05;291(4):L588-95.
    Species: Primate - Papio anubis (Olive Baboon)
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
    Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW
    Proc. Natl. Acad. Sci. U.S.A., 2005-07-11;102(29):10212-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse Angiopoietin‑1 Antibody
By Anonymous on 11/23/2015
Application: IHC Sample Tested: Adult lung Species: Mouse