Human/Mouse ATF1 Antibody

Catalog #: AF4370 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4370
AF4370-SP

Detection of Human/Mouse ATF1 by Western Blot.

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Human/Mouse ATF1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse ATF1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ATF1
Met1-Val271
Accession # P18846

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse ATF1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ATF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4370) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for ATF1 was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATF1

ATF1 is a member of the bzip family of transcription factors. ATF1, CREB, and CREM form a subfamily of bzip proteins, which respond to cAMP activation in cells by forming homo and heterodimers. These factors respond to extracellular stimulus by regulating gene expression through binding cAMP response elements (TGACGTCA).

Long Name

Activating Transcription Factor 1

Entrez Gene IDs

466 (Human); 11908 (Mouse); 315305 (Rat)

Alternate Names

activating transcription factor 1FUS/ATF-1; ATF1; cAMP-dependent transcription factor ATF-1; EWS-ATF1; Protein TREB36; TREB36; TREB36cyclic AMP-dependent transcription factor ATF-1