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Human/Mouse BACE-1 Ectodomain Antibody

Catalog #: MAB9311
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9311
MAB9311-SP
Neutralization of BACE‑1 Activity by Human/Mouse BACE‑1 Antibody.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse BACE-1 Ectodomain Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse BACE‑1 Ectodomain in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) BACE-2, recombinant mouse BACE-2, rhADAM8, rhADAM9, rhADAM10, rhADAM15, or rhTACE is observed.
Source
Monoclonal Mouse IgG1 Clone # 137626
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BACE-1
Thr22-Tyr460
Accession # P56817
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human BACE‑1 (Catalog # 931‑AS) or Recombinant Mouse BACE‑1 (Catalog # 2976-AS), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human BACE‑1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE‑1 (10 µg/mL, Catalog # 2976-AS) cleavage of the fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10 µM, Catalog # ES004). The Neutralization Dose (ND50) is typically 10 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Neutralization of BACE‑1 Activity by Human/Mouse BACE‑1 Antibody. View Larger

Neutralization of BACE‑1 Activity by Human/Mouse BACE‑1 Antibody. The cleavage of Mca-SEVNLDAEFRK(Dnp)RR-NH2(10 µM, Catalog # ES004) by Recombinant Human BACE-1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE-1 (10 µg/mL, Catalog # 2976-AS) is measured after preincubation with increasing concentrations of Mouse Anti-Human/Mouse BACE-1 Ectodomain Monoclonal Antibody (Catalog # MAB9311). The ND50 is typically 10 µg/mL.a

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BACE-1

BACE-1 (beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is the major beta secretase, and together with the gamma secretase, is responsible for generating the amyloid beta peptide (A beta ) from the amyloid precursor protein (APP) (3, 4). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta -dependent but also A beta -independent pathogenic mechanisms (5). In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins (6 - 8).

References
  1. Vassar, R. et al. (1999) Science 286:735.
  2. Yan, R. et al. (1999) Nature 402:533.
  3. Cai, H. et al. (2001) Nature Neurosci. 4:233.
  4. Roberds, S.L. et al. (2001) Human Mol. Genet. 97:1317.
  5. Rockenstein, E. et al. (2005) J. Biol. Chem. 280:32957.
  6. Li, Q and T.C. Sudhof (2004) J. Biol. Chem. 279:10542.
  7. Lichtenthaler, S.F. et al. (2003) J. Biol. Chem. 278:48713.
  8. Kitazynem, S. et al. (2005) J. Biol. Chem. 280:8589.
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 1
Entrez Gene IDs
23621 (Human); 23821 (Mouse)
Alternate Names
APP beta-secretase; ASP2; Aspartyl protease 2; BACE1; BACE-1; BACEAsp 2; beta-secretase 1 precursor variant 1; beta-secretase 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; beta-site APP-cleaving enzyme; EC 3.4.23; EC 3.4.23.46; FLJ90568; HSPC104; KIAA1149; memapsin-2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2

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Citations for Human/Mouse BACE-1 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Neuronal beta ‐amyloid generation is independent of lipid raft association of beta ‐secretase BACE1: analysis with a palmitoylation‐deficient mutant
    Authors: Kazumi Motoki, Hideaki Kume, Akiko Oda, Akira Tamaoka, Ai Hosaka, Fuyuki Kametani et al.
    Brain and Behavior
  2. LRP1 Downregulates the Alzheimer’s beta -Secretase BACE1 by Modulating Its Intraneuronal Trafficking1,2,3
    Authors: Daisuke Tanokashira, Kazumi Motoki, Seiji Minegishi, Ai Hosaka, Naomi Mamada, Akira Tamaoka et al.
    eNeuro
  3. Electrochemical Detection of Alzheimer’s Disease Biomarker, beta -Secretase Enzyme (BACE1), With One-Step Synthesized Reduced Graphene Oxide
    Authors: Jhilik Dey, Akanksha Roberts, Subhasis Mahari, Sonu Gandhi, Prem Prakash Tripathi
    Frontiers in Bioengineering and Biotechnology
  4. A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1.
    Authors: Costantini C, Ko MH, Jonas MC, Puglielli L
    Biochem. J., 2007-11-01;407(3):383-95.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  5. A cellular complex of BACE1 and gamma -secretase sequentially generates A beta from its full-length precursor
    Authors: Lei Liu, Li Ding, Matteo Rovere, Michael S. Wolfe, Dennis J. Selkoe
    Journal of Cell Biology
  6. Development of a specific ELISA to measure BACE1 levels in human tissues
    Authors: Amanda Gonzales, Boris Decourt, Aaron Walker, Rachel Condjella, Hikmet Nural, Marwan N. Sabbagh
    Journal of Neuroscience Methods
  7. &beta-Secretase1 biological markers for Alzheimer's disease: state-of-art of validation and qualification
    Authors: H Hampel, S Lista, E Vanmechele, H Zetterberg, FS Giorgi, A Galgani, K Blennow, F Caraci, B Das, R Yan, A Vergallo, Alzheimer’
    Alzheimers Res Ther, 2020-10-16;12(1):130.

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