Human/Mouse Bcl-6 Antibody

Catalog #: AF5046 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5046
AF5046-SP

Detection of Mouse and Human Bcl‑6 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Bcl-6 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Bcl-6 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Bcl-6
Glu522-Thr696
Accession # Q8CB25

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

10 µg/mL

Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Human Bcl‑6 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Bcl-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl-6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Bcl‑6 by Simple Western^TM. Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑6 at approximately 111 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Bcl‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-6

Bcl-6 (B-cell lymphoma protein 6; also ZFP51, LAZ-3 and Bcl-5) is an 80 kDa member of the Kruppel-like family of zinc finger proteins. It is expressed in germinal center B cells, neutrophils, monocytes, and immature dendritic cells, and serves as a potent transcriptional repressor of genes such as p53. Human Bcl-6 is 706 amino acids (aa) in length and contains an N-terminal BTB/POZ protein interaction domain (aa 32‑99) and a series of six C2H2-type C-terminal zinc finger domains that bind DNA (aa 518‑681). Centrally located are three PEST sequences (aa 336‑430) that undergo phosphorylation by MAPK. This increases the molecular weight of Bcl-6 to 97 kDa and induces its degradation. Human Bcl-6 is about 99% aa identical to mouse and canine Bcl-6.

Long Name

B Cell Lymphoma/Leukemia 6

Entrez Gene IDs

604 (Human); 12053 (Mouse); 303836 (Rat)

Alternate Names

B-cell CLL/lymphoma 6; B-cell lymphoma 6 protein transcript; Bcl-5; BCL5B-cell lymphoma 5 protein; Bcl6; Bcl-6; BCL6A; cys-his2 zinc finger transcription factor; LAZ3; LAZ3ZBTB27B-cell lymphoma 6 protein; lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; ZBTB27 ; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51ZNF51; zinc finger transcription factor BCL6S; ZNF51