Human/Mouse BMP-7 Antibody

Catalog #: AF354
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF354
AF354-SP

All BMP-7 Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse BMP-7 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse BMP-7 in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) BMP-2, rhBMP‑3, rhBMP‑4, rhBMP‑5, and rhBMP‑6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BMP‑7
Ser293-His431
Accession # P18075

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human BMP‑7 (Catalog # 354-BP) and Recombinant Mouse BMP-7 (Catalog # 5666-BP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-7

Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but often remains associated with the mature C-terminus. Based on in vivo and in vitro studies, BMP-7 has the potential to be secreted as a disulfide-linked mature homodimer, or particularly as a heteromeric complex that consists of two propeptides noncovalently associated with a mature disulfide-linked homodimer (5, 6). The presence of the propeptides in BMP-7 appears to stabilize the molecule and provide a docking mechanism for extracellular storage on molecules such as fibrillin-1 and -2 (5, 6). The propeptides themselves do not impart latency to the complex. BMP-7 binding to type II receptors rapidly displaces the prodomain:mature molecule interaction and has no effect on activity. But it is suggested that immobilized BMP-7 (via prodomain:fibrillin) is inactive, allowing for possible long-term storage of the molecule (6). BMP-7 interacts with the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 may also be processed into a disulfide-linked heterodimer with either BMP-2 or BMP-4. Such complexes may show increased potency and range of activity compared to BMP-7 homodimers (7 - 9). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13 - 15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16).

References

Chen, D. et al. (2004) Growth Factors 22:233.
Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
Israel, D.I. et al. (1996) Growth Factors 13:291.
Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
Zeisberg, M. et al. (2003) Nat. Med. 9:964.
Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
Chou, J. et al. (2006) J. Neurol. Sci. 240:21.

Long Name

Bone Morphogenetic Protein 7

Entrez Gene IDs

655 (Human); 12162 (Mouse); 85272 (Rat)

Alternate Names

BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1

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Citation for Human/Mouse BMP-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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