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Human/Mouse/Bovine Insulin Antibody

Catalog #: MAB1417
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1417
MAB1417-SP
Insulin in beta TC‑6 Mouse Cell Line.
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Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Bovine Insulin Antibody Summary

Species Reactivity
Human, Mouse, Bovine
Specificity
Detects bovine, human, and mouse insulin.
Source
Monoclonal Rat IgG2A Clone # 182410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Insulin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
0.5-25 µg/mL
Immersion fixed paraffin-embedded sections of human pancreas
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
beta TC‑6 mouse beta cell insulinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Insulin antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Insulin in beta TC‑6 Mouse Cell Line. Insulin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry View Larger

Insulin in Human Pancreas. Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Entrez Gene IDs
3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)
Alternate Names
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin

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Citations for Human/Mouse/Bovine Insulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. STAT3 suppression and beta-cell ablation enhance alpha-to-beta reprogramming mediated by Pdx1
    Authors: Y Wakabayash, T Miyatsuka, M Miura, M Himuro, T Taguchi, H Iida, Y Nishida, Y Fujitani, H Watada
    Scientific Reports, 2022-12-10;12(1):21419.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Morus alba L. (Sangzhi) Alkaloids Promote Insulin Secretion, Restore Diabetic beta-Cell Function by Preventing Dedifferentiation and Apoptosis
    Authors: L Lei, Y Huan, Q Liu, C Li, H Cao, W Ji, X Gao, Y Fu, P Li, R Zhang, Z Abliz, Y Liu, S Liu, Z Shen
    Frontiers in Pharmacology, 2022-03-03;13(0):841981.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of beta cell function
    Authors: MR Brown, SK Sen, A Mazzone, TK Her, Y Xiong, JH Lee, N Javeed, CS Colwell, K Rakshit, NK LeBrasseur, A Gaspar-Mai, T Ordog, AV Matveyenko
    Science Advances, 2021-12-15;7(51):eabg6856.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Tet2 Controls the Responses of beta cells to Inflammation in Autoimmune Diabetes
    Authors: J Rui, S Deng, AL Perdigoto, G Ponath, R Kursawe, N Lawlor, T Sumida, M Levine-Rit, ML Stitzel, D Pitt, J Lu, KC Herold
    Nature Communications, 2021-08-20;12(1):5074.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Glucagon-Like Peptide-1 Receptor Agonist Prevented the Progression of Hepatocellular Carcinoma in a Mouse Model of Nonalcoholic Steatohepatitis
    Authors: M Kojima, H Takahashi, T Kuwashiro, K Tanaka, H Mori, I Ozaki, Y Kitajima, Y Matsuda, K Ashida, Y Eguchi, K Anzai
    Int J Mol Sci, 2020-08-10;21(16):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Pancreatic islets seeded in a novel bioscaffold forms an organoid to rescue insulin production and reverse hyperglycemia in models of type 1 diabetes
    Authors: DM Elizondo, NZD Brandy, RLL da Silva, TR de Moura, J Ali, D Yang, MW Lipscomb
    Sci Rep, 2020-03-09;10(1):4362.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Reversal of Hyperglycemia and Suppression of Type 1 Diabetes in the NOD Mouse with Apoptotic DNA Immunotherapy� (ADi�), ADi-100
    Authors: DG Alleva, M Rezaee, L Yip, G Ren, J Rosenberg, W Concepcion, A Escher, S Shabahang, AS Thakor
    Biomedicines, 2020-03-04;8(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. VEGF-B ablation in pancreatic beta-cells upregulates insulin expression without affecting glucose homeostasis or islet lipid uptake
    Authors: FC Ning, N Jensen, J Mi, W Lindström, M Balan, L Muhl, U Eriksson, I Nilsson, D Nyqvist
    Sci Rep, 2020-01-22;10(1):923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Single-Cell Heterogeneity Analysis and CRISPR Screen Identify Key ?-Cell-Specific Disease Genes
    Authors: Z Fang, C Weng, H Li, R Tao, W Mai, X Liu, L Lu, S Lai, Q Duan, C Alvarez, P Arvan, A Wynshaw-Bo, Y Li, Y Pei, F Jin, Y Li
    Cell Rep, 2019-03-12;26(11):3132-3144.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Macrophage contact dependent and independent TLR4 mechanisms induce beta-cell dysfunction and apoptosis in a mouse model of type 2 diabetes.
    Authors: Cucak H, Mayer C, Tonnesen M, Thomsen L, Grunnet L, Rosendahl A
    PLoS ONE, 2014-03-03;9(3):e90685.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization.
    Authors: Cucak H, Grunnet L, Rosendahl A
    J Leukoc Biol, 2013-09-05;95(1):149-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Targeting VEGF-B as a novel treatment for insulin resistance and type 2 diabetes.
    Authors: Hagberg C, Mehlem A, Falkevall A, Muhl L, Fam B, Ortsater H, Scotney P, Nyqvist D, Samen E, Lu L, Stone-Elander S, Proietto J, Andrikopoulos S, Sjoholm A, Nash A, Eriksson U
    Nature, 2012-09-26;490(7420):426-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus.
    Authors: Kalis M, Bolmeson C, Esguerra JL, Gupta S, Edlund A, Tormo-Badia N, Speidel D, Holmberg D, Mayans S, Khoo NK, Wendt A, Eliasson L, Cilio CM
    PLoS ONE, 2011-12-27;6(12):e29166.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Conversion of immortal liver progenitor cells into pancreatic endocrine progenitor cells by persistent expression of Pdx-1.
    Authors: Jin CX, Li WL, Xu F, Geng ZH, He ZY, Su J, Tao XR, Ding XY, Wang X, Hu YP
    J. Cell. Biochem., 2008-05-01;104(1):224-36.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  15. Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress.
    Authors: Dowling P, O'Driscoll L, O'Sullivan F, Dowd A, Henry M, Jeppesen PB, Meleady P, Clynes M
    Proteomics, 2006-12-01;6(24):6578-87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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