Human/Mouse CCR4 Antibody

Name: Human/Mouse CCR4 Antibody
Brand: R&D Systems
SKU: AF5207
Rating: 4.5 (2 reviews)

Catalog #: AF5207
1 Citation 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5207
AF5207-SP

Detection of Human/Mouse CCR4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse CCR4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse CCR4 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CCR4
Met1-Glu39, Ala99-Lys111, Ser176-Ile205, Val268-Tyr284
Accession # P51680

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse CCR4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and EL-4 mouse lymphoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse CCR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5207) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR4

CCR4 (C-C chemokine receptor 4) is a 42 kDa member of the GPCR #1 family of transmembrane proteins. In rodent, functional CCR4 is expressed on fully differentiated CD4⁺ Th2 cells, invariant CD4⁺ NKT cells, and neurons. It is the receptor for CCL17/TARC, CCL22/MDC and CKLF1, and responds to these ligands by initiating chemotaxis. Mouse CCR4 is a 7-transmembrane protein that is 360 amino acids (aa) in length. It contains a 39 aa N-terminal extracellular domain, and a 52 aa C-terminal cyoplasmic tail that shows a poly-Ser motif between aa 343-346. Over aa sequences 1-39, 99-111, 176-205 and 268-284 collectively, mouse CCR4 shares 85% and 93% aa identity with human and rat CCR4, respectively.

Entrez Gene IDs

1233 (Human); 12773 (Mouse); 171054 (Rat); 403541 (Canine); 102120178 (Cynomolgus Monkey)

Alternate Names

C-C chemokine receptor type 4; C-C CKR-4; CC-CKR-4HGCN:14099; CCR4; CCR-4; CD194 antigen; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4MGC88293; CMKBR4k5-5; K5-5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse CCR4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1