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Human/Mouse CD117/c-kit Antibody

Catalog #: AF1356
58 Citations 7 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1356
AF1356-SP
CD117/c-kit in Mouse Embryo.
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Product Details
Citations (58)
FAQs
Supplemental Products
Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse CD117/c-kit Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CD117/c-kit in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
5 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
Lineage depleted mouse bone marrow cells
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CD117/c-kit antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

CD117/c-kit in Mouse Embryo. CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Human and Mouse CD117/c-kit antibody by Western Blot. View Larger

Detection of Human and Mouse CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9-2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human and Mouse CD117/c-kit antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse CD117/c-kit by Simple WesternTM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. 

Long Name
Stem Cell Factor Receptor
Entrez Gene IDs
3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Citations for Human/Mouse CD117/c-kit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

58 Citations: Showing 1 - 10
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  1. Immunohistochemical characterization of interstitial cells and their relationship to motor neurons within the mouse esophagus
    Authors: Bhraonain, EN;Turner, J;Hannigan, K;Sanders, K;Cobine, C;
    Research square
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells
    Authors: Zhang, X;Liu, Y;Sosa, F;Gunewardena, S;Crawford, PA;Zielen, AC;Orwig, KE;Wang, N;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue, Whole Cells, Protein
    Applications: Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Western Blot
  3. RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis
    Authors: Liu C, Wang L, Li Y et al.
    Nucleic acids research
  4. SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse
    Authors: A Uchida, K Imaimatsu, H Suzuki, X Han, H Ushioda, M Uemura, K Imura-Kish, R Hiramatsu, HM Takase, Y Hirate, A Ogura, M Kanai-Azum, A Kudo, Y Kanai
    Nature Communications, 2022-12-21;13(1):7860.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance
    Authors: T Klein-Rode, K Micklich, A Sanz-Moren, M Tost, J Calzada-Wa, T Adler, M Klaften, S Sabrautzki, B Aigner, M Kraiger, V Gailus-Dur, H Fuchs, German Mou, A Gründer, H Pahl, E Wolf, M Hrabe de A, B Rathkolb
    Scientific Reports, 2022-11-17;12(1):19793.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Uncovering the emergence of HSCs in the human fetal bone marrow by single-cell RNA-seq analysis
    Authors: Z Zheng, H He, XT Tang, H Zhang, F Gou, H Yang, J Cao, S Shi, Z Yang, G Sun, X Xie, Y Zeng, A Wen, Y Lan, J Zhou, B Liu, BO Zhou, T Cheng, H Cheng
    Cell Stem Cell, 2022-11-03;29(11):1562-1579.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice
    Authors: Peart NJ, Johnson TA, Lee S et al.
    PLOS Genetics
  8. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis
    Authors: Hirano K, Nonami Y, Nakamura Y et al.
    Communications Biology
  9. Disruption of the crypt niche promotes outgrowth of mutated colorectal tumor stem cells
    Authors: S Klingler, KS Hsu, G Hua, ML Martin, M Adileh, T Baslan, Z Zhang, PB Paty, Z Fuks, AM Brown, R Kolesnick
    JCI Insight, 2022-03-08;7(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation
    Authors: J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch
    Nature Communications, 2021-11-29;12(1):6963.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling
    Authors: IC Young, B Wu, J Andricovic, ST Chuang, R Li, A Tzatsos, RC Wu, MY Wu
    Cell Reports, 2021-11-23;37(8):110036.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis
    Authors: A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco
    Cell Reports, 2021-10-26;37(4):109885.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Regulation of intestinal immunity and tissue repair by enteric glia
    Authors: F Progatzky, M Shapiro, SH Chng, B Garcia-Cas, CH Classon, S Sevgi, A Laddach, AC Bon-Frauch, R Lasrado, M Rahim, EM Amaniti, S Boeing, K Shah, LJ Entwistle, A Suárez-Bon, MS Wilson, B Stockinger, V Pachnis
    Nature, 2021-10-20;599(7883):125-130.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo
    Authors: K Kimura, M Breitbach, FA Schildberg, M Hesse, BK Fleischman
    Bone Reports, 2021-09-29;15(0):101133.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Telocytes in the human ascending aorta: Characterization and exosome-related KLF-4/VEGF-A expression
    Authors: T Aschacher, K Schmidt, O Aschacher, E Eichmair, U Baranyi, B Winkler, M Grabenwoeg, A Spittler, F Enzmann, B Messner, J Riebandt, G Laufer, M Bergmann, M Ehrlich
    Journal of Cellular and Molecular Medicine, 2021-09-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. The combined action of CTCF and its testis-specific paralog BORIS is essential for spermatogenesis
    Authors: Rivero-Hinojosa S, Pugacheva EM, Kang S et al.
    Nature Communications
  17. Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells
    Authors: W Jia, L Kong, H Kidoya, H Naito, F Muramatsu, Y Hayashi, HY Hsieh, D Yamakawa, DK Hsu, FT Liu, N Takakura
    Nature Communications, 2021-04-09;12(1):2118.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis
    Authors: K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato
    Journal of pharmacological sciences, 2021-04-07;146(3):125-135.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  19. An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia
    Authors: S Suzuki, JR McCarrey, BP Hermann
    Cell Reports, 2021-02-16;34(7):108752.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  20. Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules
    Authors: K Imura-Kish, A Uchida, N Tsunekawa, H Suzuki, HM Takase, Y Hirate, M Kanai-Azum, R Hiramatsu, M Kurohmaru, Y Kanai
    Scientific Reports, 2021-01-13;11(1):1110.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Single-cell transcriptomics reveals zone-specific alterations of liver sinusoidal endothelial cells in cirrhosis
    Authors: T Su, Y Yang, S Lai, J Jeong, Y Jung, M McConnell, T Utsumi, Y Iwakiri
    Cellular and Molecular Gastroenterology and Hepatology, 2020-12-16;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020-10-14;11(1):5171.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  23. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development
    Authors: EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky
    Cell Stem Cell, 2020-09-17;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  24. Defective development and microcirculation of intestine in Npr2 mutant mice
    Authors: C Sogawa-Fuj, A Hanagata, Y Fujiwara, Y Ishida, H Tomiyasu, T Kunieda, H Nakatomi, M Hori
    Sci Rep, 2020-09-08;10(1):14761.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  25. PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression
    Authors: DE Ebeid, F Firouzi, CY Esquer, JM Navarrete, BJ Wang, NA Gude, MA Sussman
    Cells, 2020-08-31;9(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion during Aging
    Authors: Y Hayashi, DT Asuzu, MR Bardsley, GB Gajdos, SM Kvasha, DR Linden, RA Nagy, SA Saravanape, SA Syed, Y Toyomasu, H Yan, EN Chini, SJ Gibbons, TA Kellogg, K Khazaie, M Kuro-O, JM Espindola, MP Singh, JG Tidball, M Wehling-He, G Farrugia, T Ordog
    Cell Mol Gastroenterol Hepatol, 2020-08-07;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  27. DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors
    Authors: MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page
    Elife, 2020-07-20;9(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis
    Authors: A Heinrich, SJ Potter, L Guo, N Ratner, T DeFalco
    Cell Rep, 2020-04-14;31(2):107513.
    Species: Mouse
    Sample Types: Testes
    Applications: IF
  29. Multipotency of mouse trophoblast stem cells
    Authors: M Hou, J Han, G Li, MY Kwon, J Jiang, S Emani, ES Taglauer, JA Park, EB Choi, M Vodnala, YW Fong, SM Emani, IO Rosas, MA Perrella, X Liu
    Stem Cell Res Ther, 2020-02-13;11(1):55.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  30. Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells
    Authors: P Agarwal, S Isringhaus, H Li, AJ Paterson, J He, Á Gomariz, T Nagasawa, C Nombela-Ar, R Bhatia
    Cell Stem Cell, 2019-03-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  31. Regnase-1-mediated post-transcriptional regulation is essential for hematopoietic stem and progenitor cell homeostasis
    Authors: H Kidoya, F Muramatsu, T Shimamura, W Jia, T Satoh, Y Hayashi, H Naito, Y Kunisaki, F Arai, M Seki, Y Suzuki, T Osawa, S Akira, N Takakura
    Nat Commun, 2019-03-06;10(1):1072.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis
    Authors: S Saeidi, F Shapouri, RU de Iongh, F Casagranda, JM Sutherland, PS Western, EA McLaughlin, M Familari, GR Hime
    PLoS ONE, 2018-01-11;13(1):e0190925.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  33. Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis
    Authors: Y Kawai, A Oda, Y Kanai, R Goitsuka
    PLoS ONE, 2018-01-02;13(1):e0190702.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  34. Cardiac Nestin+ Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells
    Authors: D Xie, Y Liao, B Wu, Y Chen, W Lin, D Lu, S Gao, S Zhu, C Peng, MH Jiang
    Int Heart J, 2018-01-01;59(1):180-189.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  35. Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer
    Authors: JC Harrell, TM Shroka, BM Jacobsen
    Oncogenesis, 2017-11-27;6(11):2.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: Western Blot
  36. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. Live cell screening platform identifies PPAR? as a regulator of cardiomyocyte proliferation and cardiac repair
    Authors: A Magadum, Y Ding, L He, T Kim, MD Vasudevara, Q Long, K Yang, N Wickramasi, HV Renikunta, N Dubois, G Weidinger, Q Yang, FB Engel
    Cell Res., 2017-06-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  38. A transit-amplifying population underpins the efficient regenerative capacity of the testis
    Authors: C Carrieri, S Comazzetto, A Grover, M Morgan, A Buness, C Nerlov, D O'Carroll
    J. Exp. Med., 2017-05-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  39. Concurrent Isolation of Three Distinct Cardiac Stem Cell Populations from a Single Human Heart Biopsy
    Authors: MM Monsanto, KS White, T Kim, BJ Wang, K Fisher, K Ilves, FG Khalafalla, A Casillas, K Broughton, S Mohsin, WP Dembitsky, MA Sussman
    Circ. Res., 2017-04-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures
    Authors: MY Lee, SE Ha, C Park, PJ Park, R Fuchs, L Wei, BG Jorgensen, D Redelman, SM Ward, KM Sanders, S Ro
    PLoS ONE, 2017-04-20;12(4):e0176031.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  41. Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice
    Authors: L Luo, J Tang, K Nishi, C Yan, PU Dinh, J Cores, T Kudo, J Zhang, TS Li, K Cheng
    Circ. Res, 2017-03-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  42. Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes
    Authors: AL Feng, YY Xiang, L Gui, G Kaltsidis, Q Feng, WY Lu
    Diabetologia, 2017-03-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  43. Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice
    PLoS ONE, 2016-05-19;11(5):e0152179.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  44. Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.
    Authors: Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang Q, Lui K, Zhou B
    Cell Res, 2015-12-04;26(1):119-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  45. Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis.
    Authors: Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y, Sugiyama D
    PLoS ONE, 2015-09-21;10(9):e0138621.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  46. Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment.
    Authors: Quijada P, Hariharan N, Cubillo J, Bala K, Emathinger J, Wang B, Ormachea L, Bers D, Sussman M, Poizat C
    J Biol Chem, 2015-08-31;290(42):25411-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  47. Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium.
    Authors: Iglesias J, Cairney C, Ferrier R, McDonald L, Soady K, Kendrick H, Pringle M, Morgan R, Martin F, Smalley M, Blyth K, Stein T
    PLoS ONE, 2015-03-24;10(3):e0119718.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  48. Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth.
    Authors: Shiu J, Piazuelo M, Ding H, Czinn S, Drakes M, Banerjee A, Basappa N, Kobayashi K, Fricke W, Blanchard T
    Mucosal Immunol, 2015-01-21;8(5):1047-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  49. Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart.
    Authors: Smith A, Lewis F, Aquila I, Waring C, Nocera A, Agosti V, Nadal-Ginard B, Torella D, Ellison G
    Nat Protoc, 2014-06-19;9(7):1662-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  50. c-kit+ cells minimally contribute cardiomyocytes to the heart.
    Authors: van Berlo J, Kanisicak O, Maillet M, Vagnozzi R, Karch J, Lin S, Middleton R, Marban E, Molkentin J
    Nature, 2014-05-07;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  51. Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells.
    Authors: Toko, Haruhiro, Hariharan, Nirmala, Konstandin, Mathias, Ormachea, Lucia, McGregor, Michael, Gude, Natalie, Sundararaman, Balaji, Joyo, Eri, Joyo, Anya Y, Collins, Brett, Din, Shabana, Mohsin, Sadia, Uchida, Takafumi, Sussman, Mark A
    J Biol Chem, 2013-12-27;289(9):5348-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  52. Thymosin beta4 increases the potency of transplanted mesenchymal stem cells for myocardial repair.
    Authors: Ye L, Zhang P, Duval S, Su L, Xiong Q, Zhang J
    Circulation, 2013-09-10;128(11):S32-41.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  53. Generation of Functional Blood Vessels from a Single c-kit+ Adult Vascular Endothelial Stem Cell.
    Authors: Fang, Shentong, Wei, Jing, Pentinmikko, Nalle, Leinonen, Hannele, Salven, Petri
    PLoS Biol, 2012-10-16;10(10):e1001407.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  54. Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.
    Authors: Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y
    Blood, 2012-05-02;119(23):5429-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  55. Microenvironmental regulation of inducible nitric oxide synthase expression and nitric oxide production in mouse bone marrow-derived mast cells.
    Authors: Moon TC, Yoshimura T, Parsons T, Befus AD
    J. Leukoc. Biol., 2012-01-18;91(4):581-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  56. Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization.
    Authors: Miyamoto K, Yoshida S, Kawasumi M
    J. Exp. Med., 2011-10-17;208(11):2175-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  57. CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.
    Authors: Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ
    Int. Immunol., 2009-07-10;21(8):991-1001.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  58. Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis.
    Authors: Shin JS, Shelburne CP, Jin C, LeFurgey EA, Abraham SN
    J. Immunol., 2006-11-01;177(9):5791-800.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human/Mouse CD117/c-kit Antibody

Average Rating: 4.3 (Based on 7 Reviews)

5 Star
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4 Star
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3 Star
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Human/Mouse CD117/c-kit Antibody
By Anonymous on 10/26/2021
Application: ICC/IF Sample Tested: Nerve tissue Species: Mouse

+4°C overnight incubation 1:200, 2nd anti-goat 647 1:400

Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356
Human/Mouse CD117/c-kit Antibody
By Anonymous on 10/02/2019
Application: ICC/IF Sample Tested: Adult intestine Species: Mouse
Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356
Human/Mouse CD117/c-kit Antibody
By Anonymous on 06/12/2019
Application: WB Sample Tested: Muscle tissue Species: Mouse
Human/Mouse CD117/c-kit Antibody
By Mozhdeh Sojoodi on 05/07/2018
Application: ICC/IF Sample Tested: Liver tissue Species: Rat

1:100 dilution
RT overnight incubation
Citrate buffer heat mediated antigen retrival

Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356
Human/Mouse CD117/c-kit Antibody
By Anonymous on 04/05/2018
Application: WB Sample Tested: ES-E14TG2a Species: Mouse

The experiment was performed on ES-E14TG2a cells: two different bands of about 130 KDa (< 135 KDa) were detected.

Western Blot Human/Mouse CD117/c-kit Antibody AF1356
Human/Mouse CD117/c-kit Antibody
By Anonymous on 07/24/2017
Application: WB Sample Tested: MCF 10A human breast epithelial cell line,MCF-7 human breast cancer cell line,MDA-MB-231 human breast cancer cell line Species: Human
Human/Mouse CD117/c-kit Antibody
By Zhiheng He on 08/08/2016
Application: ICC/IF Sample Tested: Embryo tissue Species: Mouse

Mouse embryonic yolk sac tissue (E10.5), with 1:100 dilution, Alex Fluro 594 donkey anti-goat 2nd antibody with 1:400 dilution

Immunocytochemistry/Immunofluorescence Human/Mouse CD117/c-kit Antibody AF1356