Human/Mouse CXCL12/SDF-1 PerCP-conjugated Antibody

Catalog #: IC350C
1 Citation Datasheet / COA / SDS

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IC350C

Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry.

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All CXCL12/SDF-1 Antibodies

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Human/Mouse CXCL12/SDF-1 PerCP-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 79018

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of CXCL12/SDF-1 antibody in MG-63 Human Cell Line antibody by Flow Cytometry.

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Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 PerCP-conjugated Mono-clonal Antibody (Catalog # IC350C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL12/SDF-1

CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus.

Entrez Gene IDs

6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)

Alternate Names

chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human/Mouse CXCL12/SDF-1 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth
Authors: S Davidson, M Efremova, A Riedel, B Mahata, J Pramanik, J Huuhtanen, G Kar, R Vento-Torm, T Hagai, X Chen, MA Haniffa, JD Shields, SA Teichmann
Cell Rep, 2020-05-19;31(7):107628.
Species: Mouse
Sample Types: Whole Cell
Applications: Flow Cytometry