Home / EBF-2 / Human/Mouse EBF-2 Antibody

Human/Mouse EBF-2 Antibody

Catalog #: AF7006
13 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7006
AF7006-SP
Detection of Human and Mouse EBF-2 by Western Blot.
2 Images
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse EBF-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse EBF-2 in direct ELISAs. Detects mouse and human EBF-2 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse EBF-2
Arg407-Ser519
Accession # O08792
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse EBF-2 antibody by Western Blot. View Larger

Detection of Human and Mouse EBF-2 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for EBF-2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry EBF-2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

EBF-2 in Mouse Embryo. EBF-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBF-2

EBF-2 (Early B cell Factor 2; also Mmot1, OLF3 and COE2) is a 62 kDa (predicted) member of the COE family of transcription factors. It is expressed in immature osteoblasts and Purkinje cells, and in the embryo is associated with the migration of postmitotic neuroblasts. In immature osteoblasts, EBF-2 appears to upregulate OPG, suppressing osteoclast formation. And in the developing retina, EBF-2 is found in ganglion, glycinergic Amacrine and horizontal cells, possibly promoting their development over that of photoreceptor cells. Mouse EBF-2 is 575 amino acids (aa) in length. It contains one DNA-binding region with an embedded C5-type zinc‑finger motif (aa 62-238), a dimerization ITP/TIG domain (aa 253-336), and a Pro/Ser-rich transactivation domain (aa 453-534). Although considered an HLH type transcription factor, it does not contain the typical "b", or basic amino acid sequence associated with bHLH factors. EBF-2 both homodimerizes, and heterodimerizes with EBF-1 and -3. There is an alternative start site at Met23. Over aa 407-519, mouse EBF-2 is identical in aa sequence to rat EBF-2 and shares 99% aa identity with human EBF-2.

Long Name
Early B-cell Factor 2
Entrez Gene IDs
64641 (Human); 13592 (Mouse); 361060 (Rat)
Alternate Names
COE2; EBF2; EBF-2; Mmot1; O/E-3; OE-3

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse EBF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. NFIA co-localizes with PPAR gamma and transcriptionally controls the brown fat gene program
    Authors: Yuta Hiraike, Hironori Waki, Jing Yu, Masahiro Nakamura, Kana Miyake, Gaku Nagano et al.
    Nature Cell Biology
  2. Cell fate determining molecular switches and signaling pathways in Pax7-expressing somitic mesoderm
    Authors: Cheuk Wang Fung, Shaopu Zhou, Han Zhu, Xiuqing Wei, Zhenguo Wu, Angela Ruohao Wu
    Cell Discovery
  3. MLL3/MLL4 are required for CBP/p300 binding on enhancers and super-enhancer formation in brown adipogenesis
    Authors: Binbin Lai, Ji-Eun Lee, Younghoon Jang, Lifeng Wang, Weiqun Peng, Kai Ge
    Nucleic Acids Research
  4. EBF2 transcriptionally regulates brown adipogenesis via the histone reader DPF3 and the BAF chromatin remodeling complex
    Authors: Suzanne N. Shapira, Hee-Woong Lim, Sona Rajakumari, Alexander P. Sakers, Jeff Ishibashi, Matthew J. Harms et al.
    Genes & Development
  5. A single-cell atlas of human and mouse white adipose tissue
    Authors: Margo P. Emont, Christopher Jacobs, Adam L. Essene, Deepti Pant, Danielle Tenen, Georgia Colleluori et al.
    Nature
  6. Defective brown adipose tissue thermogenesis and impaired glucose metabolism in mice lacking Letmd1
    Authors: KM Choi, JH Kim, X Kong, M Isik, J Zhang, HW Lim, JC Yoon
    Cell Reports, 2021-12-14;37(11):110104.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome
    Authors: S Su, AR Guntur, DC Nguyen, SS Fakory, CC Doucette, C Leech, H Lotana, M Kelley, J Kohli, J Martino, S Sims-Lucas, L Liaw, C Vary, CJ Rosen, AC Brown
    Cell Rep, 2018-12-11;25(11):3215-3228.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting
    Authors: S Elattar, M Dimri, A Satyanaray
    FASEB J., 2018-03-23;0(0):fj201701465RR.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning
    Authors: M Patil, BK Sharma, S Elattar, J Chang, S Kapil, J Yuan, A Satyanaray
    Diabetes, 2017-03-07;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. A Multi-step Transcriptional and Chromatin State Cascade Underlies Motor Neuron Programming from Embryonic Stem Cells
    Authors: Silvia Velasco
    Cell Stem Cell, 2016-12-08;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation
  11. A long noncoding RNA transcriptional regulatory circuit drives thermogenic adipocyte differentiation.
    Authors: Zhao X, Li S, Wang G, Yu Q, Lin J
    Mol Cell, 2014-07-04;55(3):372-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  12. Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons
    Authors: ES Liau, S Jin, YC Chen, WS Liu, M Calon, S Nedelec, Q Nie, JA Chen
    Oncogene, 2023-01-03;14(1):46.
  13. EBF2 promotes the recruitment of beige adipocytes in white adipose tissue
    Authors: Rachel R. Stine, Suzanne N. Shapira, Hee-Woong Lim, Jeff Ishibashi, Matthew Harms, Kyoung-Jae Won et al.
    Molecular Metabolism

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse EBF-2 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse EBF-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse EBF-2 Antibody
By Anonymous on 07/30/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Human fibroblast Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse EBF-2 Antibody AF7006