Human/Mouse Fatty Acid Synthase/FASN Antibody

Catalog #: MAB5927 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5927
MAB5927-SP

Detection of Human and Mouse Fatty Acid Synthase/FASN by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Fatty Acid Synthase/FASN Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Fatty Acid Synthase/FASN in Western blots.

Source

Monoclonal Mouse IgG2b Clone # 647113

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

20 µg/mL

HepG2 human hepatocellular carcinoma cell line

Knockout Validated

Fatty Acid Synthase/FASN is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Fatty Acid Synthase/FASN knockout HEK293T cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Fatty Acid Synthase/FASN by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB5927) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Fatty Acid Synthase/FASN at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Knockout Validated

Western Blot Shows Human Fatty Acid Synthase/FASN Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human Fatty Acid Synthase/FASN Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Fatty Acid Synthase/FASN knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB5927) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Fatty Acid Synthase/FASN at approximately 275 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Fatty Acid Synthase/FASN by Simple Western^TM. Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Fatty Acid Synthase/FASN at approximately 213 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB5927). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Fatty Acid Synthase/FASN

FASN (Fatty Acid Synthase; also FAS) is a cytosolic 270-280 kDa member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes, and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA, and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length ands contains multiple domains, including a beta -ketoacyl synthase domain (aa 1-414), an acyl and malonyl transferase domain (aa 429-817), an enoyl reductase domain (aa 1563-1863), a beta -ketoacyl reductase domain (aa 1864-2118) and a thioesterase domain (aa 2207‑2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 9-212, human FASN shares 92% aa identity with mouse FASN.

Entrez Gene IDs

2194 (Human); 14104 (Mouse)

Alternate Names

EC 2.3.1; EC 2.3.1.85; EC 6.3.3.1; FASN; FASshort chain dehydrogenase/reductase family 27X, member 1; Fatty Acid Synthase; MGC14367; MGC15706; OA-519; SDR27X1