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Human/Mouse FLIP Antibody

Catalog #: AF821
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF821
AF821-SP
Detection of Human/Mouse FLIP by Western Blot.
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Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse FLIP Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse FLIP in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
KLH-coupled mouse FLIP synthetic peptide
LKQGRRRPLVDLHVELMDKC
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse FLIP antibody by Western Blot. View Larger

Detection of Human/Mouse FLIP by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse FLIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF821) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FLIP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLIP

FLIP (Flice-like Inhibitory Protein), also known as I-FLICE, CASPER, and FLAME-1, is an apoptosis inhibitory protein with architecture similar to that of Caspases-8 and -10. Both of the FLIP isoforms, Long (L) and Short (S), contain two death effector domains (DED). FLIP(L) has a C-terminal Caspase-like protease domain which lacks both a catalytic active site and residues that form a substrate-binding pocket. FLIP(S) and FLIP(L) interact with the adaptor molecule FADD, and potently inhibit apoptosis initiated by Fas Ligand/TNF signaling pathways.

Long Name
FLICE Inhibitory Proteins
Entrez Gene IDs
8837 (Human); 12633 (Mouse)
Alternate Names
CASHFADD-like antiapoptotic molecule 1; CASP8 and FADD-like apoptosis regulator; CASP8AP1; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; CASPER; CasperFLAME1; Cellular FLICE-like inhibitory protein; CFLAR; c-FLIPR; c-FLIPS; CLARPMACH-related inducer of toxicity; FADD-like apoptotic molecule; FLAME; FLAME-1; FLIP; I-FLICE; I-FLICEMRITc-FLIPFLIPc-FLIPL; Inhibitor of FLICE; usurpin beta; Usurpin

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Citations for Human/Mouse FLIP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells
    Authors: J Huangfu, C Hao, Z Wei, IM Wormstone, H Yan, X Fan
    Cell Death & Disease, 2021-04-09;12(4):386.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Forefront: MiR-34a-Knockout Mice with Wild Type Hematopoietic Cells, Retain Persistent Fibrosis Following Lung Injury
    Authors: R Bulvik, M Biton, N Berkman, R Breuer, SB Wallach-Da
    Int J Mol Sci, 2020-03-23;21(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  3. Tissue Inhibitor of Metalloproteinase-3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells
    Authors: WR English, H Ireland-Ze, AH Baker, TD Littlewood, MR Bennett, G Murphy
    PLoS ONE, 2018-04-04;13(4):e0195116.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Infrared radiation confers resistance to UV-induced apoptosis via reduction of DNA damage and upregulation of antiapoptotic proteins.
    Authors: Jantschitsch C, Majewski S, Maeda A, Schwarz T, Schwarz A
    J. Invest. Dermatol., 2008-11-27;129(5):1271-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis.
    Authors: Stefanescu R, Bassett D, Modarresi R, Santiago F, Fakruddin M, Laurence J
    Blood, 2008-03-13;112(2):340-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Low concentration of interleukin-1beta induces FLICE-inhibitory protein-mediated beta-cell proliferation in human pancreatic islets.
    Authors: Maedler K, Schumann DM, Sauter N, Ellingsgaard H, Bosco D, Baertschiger R, Iwakura Y, Oberholzer J, Wollheim CB, Gauthier BR, Donath MY
    Diabetes, 2006-10-01;55(10):2713-22.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  7. Expression of Fas but not Fas ligand on fetal pig beta cells.
    Authors: Bai L, Maedler K, Donath M, Tuch BE
    Xenotransplantation, 2004-09-01;11(5):426-35.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC
  8. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Authors: Sade H, Krishna S, Sarin A
    J. Biol. Chem., 2003-10-28;279(4):2937-44.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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