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Human/Mouse FoxP2 Antibody

Catalog #: AF5647
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5647
AF5647-SP
Detection of Human FoxP2 by Western Blot.
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Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse FoxP2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse FoxP2 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FoxP2 isoform 1
Ala640-Glu715
Accession # O15409
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human FoxP2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A172 human glioblastoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for FoxP2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry FoxP2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

FoxP2 in HeLa Human Cell Line. FoxP2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxP2

FoxP2 (Forkhead box protein P2; also CAGH44) is an 85‑95 kDa member of the winged helix transcription factor gene family. It is a transcriptional repressor that is expressed principally in gut and lung epithelium, and in neurons involved in speech and language. Human FoxP2 is 715 amino acids (aa) in length. It contains a huge poly-Gln region (aa 123‑231), a C2H2 Zn-finger domain (aa 346‑371), a Leu-zipper segment (aa 388‑409) and a forkhead DNA binding domain (aa 504‑594). FoxP2 both homodimerizes and heterodimerizes with FoxP1 and P4, and binds CTBP1. There are multiple splice variants. There is an alternate start site at Met93, an 11 aa substitution for aa 57‑715, a 25 aa insert after Gln86, an 11 aa substitution for aa 133‑715, and a ten aa substitution for aa 423‑715. Over aa 587‑715, human and mouse FoxP2 are (100%) identical in aa sequence.

Long Name
Forkhead Box P2
Entrez Gene IDs
93986 (Human); 114142 (Mouse); 500037 (Rat)
Alternate Names
CAG repeat protein 44; CAGH44; CAGH44TNRC10forkhead box protein P2; DKFZp686H1726; forkhead box P2; forkhead/winged-helix transcription factor; FoxP2; SPCH1; TNRC10; trinucleotide repeat containing 10; Trinucleotide repeat-containing gene 10 protein

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Citations for Human/Mouse FoxP2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Cerebellar nuclei neurons projecting to the lateral parabrachial nucleus modulate classical fear conditioning
    Authors: KD Hwang, J Baek, HH Ryu, J Lee, HG Shim, SY Kim, SJ Kim, YS Lee
    Cell Reports, 2023-03-22;42(4):112291.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
    Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
    Cell Rep, 2020-11-24;33(8):108425.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. The cellular basis of distinct thirst modalities
    Authors: AH Pool, T Wang, DA Stafford, RK Chance, S Lee, J Ngai, Y Oka
    Nature, 2020-10-14;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Chemosensory modulation of neural circuits for sodium appetite
    Authors: S Lee, V Augustine, Y Zhao, H Ebisu, B Ho, D Kong, Y Oka
    Nature, 2019-03-27;568(7750):93-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Efferent projections of excitatory and inhibitory preB�tzinger complex neurons
    Authors: CF Yang, JL Feldman
    J. Comp. Neurol., 2018-03-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Expression of the transcription factor FOXP2 in brainstem respiratory circuits of adult rat is restricted to upper-airway pre-motor areas
    Authors: D Stani?, RR Dhingra, M Dutschmann
    Respir Physiol Neurobiol, 2018-02-04;250(0):14-18.
    Species: Rat, Western Blot
    Sample Types: Cell Lysates, Whole Tissues
    Applications: IHC, Western Blot
  7. Barrington's nucleus: Neuroanatomic landscape of the mouse pontine micturition center
    Authors: AM Verstegen, V Vanderhors, PA Gray, ML Zeidel, JC Geerling
    J. Comp. Neurol, 2017-04-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. K�lliker-Fuse GABAergic and glutamatergic neurons project to distinct targets
    Authors: Joel Geerling
    J. Comp. Neurol, 2017-03-14;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  9. HSD2 neurons in the hindbrain drive sodium appetite
    Nat. Neurosci, 2016-12-05;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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