Home / Frizzled-2 / Human/Mouse Frizzled-2 Antibody

Human/Mouse Frizzled-2 Antibody

Catalog #: MAB1307
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1307-050
MAB1307-500
MAB1307-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Frizzled-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Frizzled-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse (rm) Frizzled-1, -3, -4, -6, -7, -8, or rhFrizzled-5.
Source
Monoclonal Rat IgG2A Clone # 202102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-2
Gln29-Leu168
Accession # Q9JIP6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Frizzled‑2 Fc Chimera (Catalog # 1307-FZ)
Immunoprecipitation

Gujral, T. et al. (2014) Cell 159:844.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-2

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 AA and contains 10 invariant cysteines (1). Mouse Frizzled-2 shows 100% amino acid identity to human and rat Frizzled-2 in the CRD region. Frizzled-2 expression is greater in embryonic than adult tissues, with heart, brain, lung, kidney and gut showing the highest levels (2). In addition, Frizzled-2 is expressed during migration and proliferation of neural crest cells that populate the heart and great arteries (3). A non-canonical Frizzled-2 pathway has been shown to regulate Epithelial-Mesenchymal Transition (EMT) and metastasis (4). Secreted frizzled related proteins (sFRPs), antagonists of the Wnt pathway, have been shown to interact with both Wnt ligands and Frizzled receptors (5).

References
  1. Wang, Y. et al. (1996) J. Biol. Chem 271:4468.
  2. Malik, T. and R. Shivdasani (2000) Biochem. J. 349:829.
  3. van Gijn, M. et al. (2001) Anat. Embryol. 203:185.
  4. Gujral, T. et al. (2014) Cell 159:844.
  5. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
Entrez Gene IDs
2535 (Human); 57265 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 2; frizzled homolog 2 (Drosophila); Frizzled2; Frizzled-2; Fz2; fz-2; FZD2; fzE2; hFz2

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Citations for Human/Mouse Frizzled-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Bioengineered BERA-Wnt5a siRNA targeting Wnt5a/FZD2 signaling suppresses advanced prostate cancer tumor growth and enhances enzalutamide treatment
    Authors: AC Gao, S Ning, C Liu, W Lou, JC Yang, AP Lombard, LS D'Abronzo, N Batra, AM Yu, AR Leslie, M Sharifi, CP Evans
    Molecular Cancer Therapeutics, 2022-10-07;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Stably overexpressed human Frizzled-2 signals through the beta-catenin pathway and does not activate Ca2+-mobilization in Human Embryonic Kidney 293 cells.
    Authors: Verkaar F, van Rosmalen JW, Smits JF, Blankesteijn WM, Zaman GJ
    Cell. Signal., 2008-09-25;21(1):22-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development

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