Human/Mouse GATA-3 Antibody

Catalog #: MAB6330
10 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6330
MAB6330-SP

Detection of Human and Mouse GATA‑3 by Western Blot.

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All GATA-3 Antibodies

Product Details

Citations (10)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GATA-3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse GATA-3 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 634913

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Dual RNAscope ISH-IHC

5-25 µg/mL

See below

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse GATA‑3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Moue Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for full length (FL) GATA-3 at approximately 52 kDa and the splice form (SF) found in MCF-7 cells at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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GATA‑3 in MCF‑7 Human Cell Line. GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog # MAB6330) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human GATA‑3 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. Specific bands were detected for GATA‑3 at approximately 53 (splice variant) and 61 kDa (full length) as indicated, using 10 µg/mL of Mouse Anti-Human/Mouse GATA‑3 Monoclonal Antibody (Catalog # MAB6330). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Dual RNAscope ISH-IHC Compatible

GATA-3 antibody in Human Breast Cancer Tissue Using Dual RNAscope® ISH and IHC.

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GATA-3 in Human Breast Cancer Tissue Using Dual RNAscope® ISH and IHC. GATA-3 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer tissue probed with ACD RNAScope® Probe (Catalog # 403551) followed by immunohistochemistry using R&D Systems Mouse Anti-Human/Mouse GATA-3 Monoclonal Antibody (Catalog# MAB6330) at 5ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001). Tissue was stained using ACD RNAscope® 2.5 HD Duplex Detection Reagents (Catalog # 322500).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Entrez Gene IDs

2625 (Human); 14462 (Mouse)

Alternate Names

131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse GATA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Annexin A1 is a cell-intrinsic metalloregulator of zinc in human ILC2s
Authors: Irie, M;Kabata, H;Sasahara, K;Kurihara, M;Shirasaki, Y;Kamatani, T;Baba, R;Matsusaka, M;Koga, S;Masaki, K;Miyata, J;Araki, Y;Kikawada, T;Kabe, Y;Suematsu, M;Yamagishi, M;Uemura, S;Moro, K;Fukunaga, K;
Cell reports
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation
Authors: F Caso, A Saviano, M Tasso, F Raucci, N Marigliano, S Passavanti, P Frallonard, R Ramonda, V Brancaleon, M Bucci, R Scarpa, L Costa, F Maione
Autoimmunity reviews, 2022-10-01;21(12):103207.
Species: Human
Sample Types: Synovial Fluid
Applications: Western Blot
Predictive role of endometrial T-bet/GATA3 ratio during mid-luteal phase for live birth in patients undergoing in vitro fertilization: A retrospective observational study
Authors: Y Li, S Yu, C Huang, L Diao, C Chen, W Liu, R Lian, M Mo, C Du, F Liu, Y Zeng
Journal of reproductive immunology, 2021-12-16;149(0):103465.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Generation of human blastocyst-like structures from pluripotent stem cells
Authors: Y Fan, Z Min, S Alsolami, Z Ma, E Zhang, W Chen, K Zhong, W Pei, X Kang, P Zhang, Y Wang, Y Zhang, L Zhan, H Zhu, C An, R Li, J Qiao, T Tan, M Li, Y Yu
Cell Discovery, 2021-09-07;7(1):81.
Species: Human
Sample Types: Spheroids
Applications: IHC
Protein O-GlcNAcylation Promotes Trophoblast Differentiation at Implantation
Authors: PT Ruane, CMJ Tan, DJ Adlam, SJ Kimber, DR Brison, JD Aplin, M Westwood
Cells, 2020-10-06;9(10):.
Species: Mouse
Sample Types: Embryo, Whole Cells
Applications: ICC, IHC
IL-13 is produced by tumor cells in Breast Implant Associated Anaplastic Large Cell Lymphoma: Implications for pathogenesis
Authors: ME Kadin, J Morgan, H Xu, AL Epstein, D Sieber, BA Hubbard, WP Adams, CE Bacchi, JCS Goes, MW Clemens, L Jeffrey Me, RN Miranda
Hum. Pathol., 2018-04-22;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Increased TREM-2 expression on the subsets of CD11c(+) cells in the lungs and lymph nodes during allergic airway inflammation
Authors: SC Hall, DK Agrawal
Sci Rep, 2017-09-19;7(1):11853.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
ZNF503/Zpo2 drives aggressive breast cancer progression by down-regulation of GATA3 expression
Authors: P Shahi, CY Wang, DA Lawson, EM Slorach, A Lu, Y Yu, MD Lai, H Gonzalez V, Z Werb
Proc. Natl. Acad. Sci. U.S.A, 2017-03-03;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Analysis of histological and immunological parameters of metastatic lymph nodes from colon cancer patients reveals that T-helper 1 type immune response is associated with improved overall survival
Authors: Joseph Klausner
Medicine (Baltimore), 2016-11-01;95(45):e5340.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Methylation of Gata3 protein at Arg-261 regulates transactivation of the Il5 gene in T helper 2 cells.
Authors: Hosokawa H, Kato M, Tohyama H, Tamaki Y, Endo Y, Kimura M, Tumes D, Motohashi S, Matsumoto M, Nakayama K, Tanaka T, Nakayama T
J Biol Chem, 2015-04-10;290(21):13095-103.
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP