Human/Mouse Gephyrin/GPHN Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Gephyrin/GPHN

Gephyrin (GPHN, from gamma epsilon φυρ alpha, the Greek word for bridge; also known as molybdopterin adenyltransferase) is a 90-94 kDa member of both the MoaB and MoeA families of proteins. It has limited expression, being found in hepatocytes and neurons. In neurons, gephyrin is a postsynaptic density cytoplasmic protein that interacts with the subunits of glycine and GABA-A receptors. This interaction is mediated by the ability of gephyrin to form hexameric lattices that act as scaffolds that link the subunits of the two inhibitory receptors to the cell's underlying tubulin network. In addition, the gephyrin lattice may also interact with NLG-1 and -2, suggesting a role for gephyrin in the regulation of glutamatergic synapses. Gephyrin is also known to participate in the synthesis of molybdenum cofactor. This cofactor binds molybdenum, making it available to sulfite and aldehyde reductase. Human gephyrin is 736 amino acids (aa) in length. It contains a 181 aa N-terminal G-domain that possesses MPT Mo-transferase activity (aa 14-166) and a homotrimer interface motif, followed by a 420 aa C-terminal region (aa 319-738) that contains an MPT adenyltransferase domain (aa 326-736) with an embedded homodimerization motif. There are at least five utilized Ser/Thr phosphorylation sites. Multiple splice variants exist, and represent peptide insertions at multiple sites. There is a 13 aa insertion after Glu98, a 33 aa insertion after Ser243, and 15, 19, 21 and 24 aa insertions after Lys288. Over aa 2-188, human and mouse Gephyrin are identical in amino acid sequence.

Product Datasheets

Related Research Areas

• GABA Receptors
• Neurotransmitter Receptors on VSMC
• Synaptic Proteins