Human/Mouse Glucagon Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Glucagon in beta TC-6 Mouse Cell Line. Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Glucagon in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glucagon
Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.
Product Datasheets
Citations for Human/Mouse Glucagon Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Roles of FoxM1-driven basal beta-cell proliferation in maintenance of beta-cell mass and glucose tolerance during adulthood
Authors: M Kohata, J Imai, T Izumi, J Yamamoto, Y Kawana, A Endo, H Sugawara, J Seiko, H Kubo, H Kawamura, T Sato, S Osaka, Y Munakata, Y Asai, S Kodama, K Takahashi, K Kaneko, H Katagiri
Journal of diabetes investigation, 2022-07-15;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model
Authors: Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann
Cell Reports, 2022-04-12;39(2):110670.
Species: Mouse
Sample Types: Tissue Culture Supernates
Applications: Neutralization -
Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans
Authors: XX Yu, WL Qiu, L Yang, YC Wang, MY He, D Wang, Y Zhang, LC Li, J Zhang, Y Wang, CR Xu
Cell Research, 2021-03-10;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Direct suppression of human islet dedifferentiation, progenitor genes, but not epithelial to mesenchymal transition by liraglutide
Authors: P Rattanaamn, YM Roshorm, C Wilasrusme, N Proprom, B Ongphiphad, SC Talchai
Heliyon, 2020-09-15;6(9):e04951.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Abnormal neutrophil signature in the blood and pancreas of presymptomatic and symptomatic type 1 diabetes
Authors: F Vecchio, N Lo Buono, A Stabilini, L Nigi, MJ Dufort, S Geyer, PM Rancoita, F Cugnata, A Mandelli, A Valle, P Leete, F Mancarella, PS Linsley, L Krogvold, KC Herold, HE Larsson, SJ Richardson, NG Morgan, K Dahl-Jørge, G Sebastiani, F Dotta, E Bosi, M Battaglia
JCI Insight, 2018-09-20;3(18):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate.
Authors: Gomez D, O'Driscoll M, Sheets T, Hruban R, Oberholzer J, McGarrigle J, Shamblott M
PLoS ONE, 2015-08-19;10(8):e0133862.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
Authors: Kornete, Mara, Beauchemin, Hugues, Polychronakos, Constant, Piccirillo, Ciriaco
Autoimmunity, 2013-02-04;46(4):259-68.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Stem cells derived from human fetal membranes display multilineage differentiation potential.
Authors: Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U
Biol. Reprod., 2007-05-09;77(3):577-88.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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