Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody Summary
Arg575-Asn826
Accession # Q16665.1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a APC-conjugated Monoclonal Antibody (Catalog # IC1935A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HIF-1 alpha/HIF1A
The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
Product Datasheets
Citations for Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Itaconate ameliorates autoimmunity by modulating T cell imbalance via metabolic and epigenetic reprogramming
Authors: K Aso, M Kono, M Kanda, Y Kudo, K Sakiyama, R Hisada, K Karino, Y Ueda, D Nakazawa, Y Fujieda, M Kato, O Amengual, T Atsumi
Nature Communications, 2023-02-27;14(1):984.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Hypoxia induces HIF1alpha-dependent epigenetic vulnerability in triple negative breast cancer to confer immune effector dysfunction and resistance to anti-PD-1 immunotherapy
Authors: S Ma, Y Zhao, WC Lee, LT Ong, PL Lee, Z Jiang, G Oguz, Z Niu, M Liu, JY Goh, W Wang, MA Bustos, S Ehmsen, A Ramasamy, DSB Hoon, HJ Ditzel, EY Tan, Q Chen, Q Yu
Nature Communications, 2022-07-15;13(1):4118.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Endogenous oxidized phospholipids reprogram cellular metabolism and boost hyperinflammation
Authors: M Di Gioia, R Spreafico, JR Springstea, MM Mendelson, R Joehanes, D Levy, I Zanoni
Nat. Immunol., 2019-11-25;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor
Authors: Q Li, D Li, X Zhang, Q Wan, W Zhang, M Zheng, L Zou, C Elly, JH Lee, YC Liu
Immunity, 2018-02-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody
Average Rating: 5 (Based on 2 Reviews)
Have you used Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
