Home / Histone Deacetylase 8/HDAC8 / Human/Mouse Histone Deacetylase 8/
HDAC8 Biotinylated Antibody

Human/Mouse Histone Deacetylase 8/
HDAC8 Biotinylated Antibody

Catalog #: BAF4359 Datasheet / COA / SDS
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BAF4359
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Human/Mouse Histone Deacetylase 8/
HDAC8 Biotinylated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Histone Deacetylase 8/HDAC8 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Histone Deacetylase 8/HDAC8 isoform 1
Met1-Val377
Accession # ACT64512
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Histone Deacetylase 8/HDAC8 (Catalog # 4359-DA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histone Deacetylase 8/HDAC8

Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases (HDACs) (1, 2). HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells (3). HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer (4). Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation (5, 6). Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor trichostatin A (1).

References
  1. Hu, E. et al. (2000) J. Biol. Chem. 275:15254.
  2. Annemieke, J et al. (2003) Biochem. J. 370:737.
  3. Gray, S. and T. Ekstrom (2001) Exp. Cell Res. 262:75.
  4. Yang, X. and S. Gregoire (2005) Mol. Cel. Biol. 25:2873.
  5. Waltregny, D. et al. (2004) Am. J. Pathol. 165:553.
  6. De Levak, L. et al. (2006) Am. J. Surg. Pathol. 30:319.
Entrez Gene IDs
55869 (Human); 70315 (Mouse); 363481 (Rat)
Alternate Names
EC 3.5.1.98; HD8; HDAC8; HDACL1; Histone Deacetylase 8; histone deacetylase-like 1; RPD3

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HDAC8 Biotinylated Antibody

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