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Human/Mouse IGF-I R/IGF1R Antibody

Catalog #: MAB391
17 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB391-SP
MAB391-100
MAB391-500
Detection of Human IGF-I R/IGF1R by Western Blot.
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Product Details
Citations (17)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse IGF-I R/IGF1R Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human IGF-I R/IGF1R in sandwich ELISAs and Western blots. Detects mouse IGF-I R/IGF1R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF‑II, rhIL‑3 R alpha, rhIL‑9 R, and rhTGF‑ beta  RII.
Source
Monoclonal Mouse IgG1 Clone # 33255
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R/IGF1R
Glu31-Asn932
Accession # P08069
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Human IGF-I R/IGF1R Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IGF-I R/IGF1R Biotinylated Antibody (Catalog # BAF391)

Standard: Recombinant Human IGF-I R/IGF1R Protein, CF (Catalog # 391-GR)

Neutralization
Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. At 11 µg/mL, this antibody will neutralize approximately 50-75% of the bioactivity due to 6 ng/mL Recombinant Human IGF‑I.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IGF-I R/IGF1R antibody by Western Blot. View Larger

Detection of Human IGF-I R/IGF1R by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, SK-Mel-28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGF-I R/IGF1R at approximately 275 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.

Flow Cytometry Detection of IGF-I R/IGF1R antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IGF-I R/IGF1R in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry IGF-I R/IGF1R antibody in MCF-7 and HDLM Human Cell Lines by Immunocytochemistry (ICC). View Larger

IGF-I R/IGF1R in MCF‑7 and HDLM Human Cell Lines. IGF-I R/IGF1R was detected in immersion fixed MCF-7 human breast cancer cell line (positive staining; left panel) and HDLM human Hodgkin's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane in MCF-7 cell line. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry IGF-I R/IGF1R antibody in Mouse Heart by Immunohistochemistry (IHC-P). View Larger

IGF-I R/IGF1R in Mouse Heart. IGF-I R/IGF1R was detected in perfusion fixed paraffin-embedded sections of mouse heart using Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF-I R/IGF1R Antibody. View Larger

Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF-I R/IGF1R Antibody. Recombinant Human (rh) IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by rhIGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391). At 11 µg/mL, this antibody will neutralize 50-75% rhIGF-1 induced activity.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I R/IGF1R

Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

Long Name
Insulin-like Growth Factor I Receptor
Entrez Gene IDs
3480 (Human)
Alternate Names
CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

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Citations for Human/Mouse IGF-I R/IGF1R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancer
    Authors: T Khan, AM Seddon, AG Dalgleish, S Khelwatty, N Ioannou, S Mudan, H Modjtahedi
    Oncol Rep, 2020-10-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs
    Authors: J Li, E Weber, S Guth-Gunde, M Schuleit, A Kuttler, C Halleux, N Accart, A Doelemeyer, A Basler, B Tigani, K Wuersch, M Fornaro, M Kneissel, A Stafford, BR Freedman, DJ Mooney
    Adv Healthc Mater, 2018-02-14;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Capture
  3. IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer
    Authors: Y Neuzillet, E Chapeaubla, C Krucker, L De Koning, T Lebret, F Radvanyi, I Bernard-Pi
    BMC Cancer, 2017-09-07;17(1):636.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs
    Authors: Helmout Modjtahedi
    Int. J. Oncol., 2016-09-05;49(5):1825-1838.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  5. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes
    Authors: M Gschweitl, A Ulbricht, CA Barnes, RI Enchev, I Stoffel-St, N Meyer-Scha, J Huotari, Y Yamauchi, UF Greber, A Helenius, M Peter
    Elife, 2016-03-23;5(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.
    Authors: Garwood C, Ratcliffe L, Morgan S, Simpson J, Owens H, Vazquez-Villasenor I, Heath P, Romero I, Ince P, Wharton S
    Mol Brain, 2015-08-22;8(0):51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Targeting both IGF-1R and mTOR synergistically inhibits growth of renal cell carcinoma in vitro.
    Authors: Cardillo T, Trisal P, Arrojo R, Goldenberg D, Chang C
    BMC Cancer, 2013-04-01;13(0):170.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators.
    Authors: Suh H, Zhao M, Derico L, Choi N, Lee S
    J Neuroinflammation, 2013-03-12;10(0):37.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Proinflammatory actions of visfatin/nicotinamide phosphoribosyltransferase (Nampt) involve regulation of insulin signaling pathway and Nampt enzymatic activity.
    Authors: Jacques C, Holzenberger M, Mladenovic Z
    J. Biol. Chem., 2012-03-07;287(18):15100-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Expression of the IGF axis is decreased in local prostate cancer but enhanced after benign prostate epithelial differentiation and TGF-beta treatment.
    Authors: Massoner P, Ladurner Rennau M, Heidegger I, Kloss-Brandstatter A, Summerer M, Reichhart E, Schafer G, Klocker H
    Am. J. Pathol., 2011-10-06;179(6):2905-19.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases.
    Authors: Wang N, Fallavollita L, Nguyen L, Burnier J, Rafei M, Galipeau J, Yakar S, Brodt P
    Mol. Ther., 2009-04-14;17(7):1241-9.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  12. Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes.
    Authors: Back K, Arnqvist HJ
    Growth Horm. IGF Res., 2008-08-09;19(2):101-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  13. Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
    Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
    Leuk. Res., 2008-03-04;32(8):1244-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Mesenchymal stem cell abnormalities in patients with multiple myeloma.
    Authors: Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ
    Leuk. Lymphoma, 2007-10-01;48(10):2032-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007-04-19;25(8):1862-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Insulin-like growth factor (IGF) 2 stimulates steroidogenesis and mitosis of bovine granulosa cells through the IGF1 receptor: role of follicle-stimulating hormone and IGF2 receptor.
    Authors: Spicer LJ, Aad PY
    Biol. Reprod., 2007-03-14;77(1):18-27.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Western Blot
  17. The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle.
    Authors: Quirk SM, Cowan RG, Harman RM
    J. Endocrinol., 2006-06-01;189(3):441-53.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Neutralization

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Human/Mouse IGF-I R/IGF1R Antibody
By Anonymous on 05/20/2022
Application: WB Sample Tested: Heart tissue Species: Mouse
Western Blot Human/Mouse IGF-I R/IGF1R Antibody MAB391